Overcoming hydrolysis of raw corn starch under industrial conditions with Bacillus licheniformis ATCC 9945a α-amylase

α-Amylase from Bacillus licheniformis ATCC 9945a ( Bli Amy) was proven to be very efficient in hydrolysis of granular starch below the temperature of gelatinization. By applying two-stage feeding strategy to achieve high-cell-density cultivation of Escherichia coli and extracellular production of Bl...

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Bibliographic Details
Published in:Applied microbiology and biotechnology 2016-03, Vol.100 (6), p.2709-2719
Main Authors: Sokarda Slavic, Marinela, Pesic, Milja, VujAeic, Zoran, Bozic, Natasa
Format: Article
Language:English
Subjects:
alpha-Amylases - genetics
alpha-Amylases - metabolism
Bacillus - enzymology
Bacillus licheniformis
Biomedical and Life Sciences
Biotechnologically Relevant Enzymes and Proteins
Biotechnology
Biotransformation
Escherichia coli
Escherichia coli - genetics
Escherichia coli - growth & development
Escherichia coli - metabolism
Hydrolysis
Kinetics
Life Sciences
Microbial Genetics and Genomics
Microbiology
Recombinant Proteins - genetics
Recombinant Proteins - metabolism
Starch - metabolism
Zea mays - metabolism
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Summary:α-Amylase from Bacillus licheniformis ATCC 9945a ( Bli Amy) was proven to be very efficient in hydrolysis of granular starch below the temperature of gelatinization. By applying two-stage feeding strategy to achieve high-cell-density cultivation of Escherichia coli and extracellular production of Bli Amy, total of 250.5 U/mL (i.e. 0.7 g/L) of enzyme was obtained. Thermostability of amylase was exploited to simplify purification. The hydrolysis of concentrated raw starch was optimized using response surface methodology. Regardless of raw starch concentration tested (20, 25, 30 %), Bli Amy was very effective, achieving the final hydrolysis degree of 91 % for the hydrolysis of 30 % starch suspension after 24 h. The major A-type crystalline structure and amorphous domains of the starch granule were degraded at the same rates, while amylose-lipid complexes were not degraded. Bli Amy presents interesting performances on highly concentrated solid starch and could be of value for starch-consuming industries while response surface methodology (RSM) could be efficiently applied for the optimization of the hydrolysis.
ISSN:0175-7598
1432-0614
DOI:10.1007/s00253-015-7101-4