Antifungal activity of Rubus chingii extract combined with fluconazole against fluconazole-resistant Candida albicans

ABSTRACT This study aimed to investigate the antifungal activity of Rubus chingii extract in combination with fluconazole (FLC) against FLC‐resistant Candida albicans 100 in vitro. A R. chingii extract and FLC‐resistant C. albicans fungus suspension were prepared. The minimum inhibitory concentratio...

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Published in:Microbiology and immunology 2016-02, Vol.60 (2), p.82-92
Main Authors: Han, Bing, Chen, Jia, Yu, Yi-qun, Cao, Yong-bing, Jiang, Yuan-ying
Format: Article
Language:English
Subjects:
Antifungal Agents - pharmacology
Apoptosis - drug effects
Birth weight
Candida albicans
Candida albicans - cytology
Candida albicans - drug effects
Candida albicans - growth & development
Candida albicans - metabolism
Cell Cycle - drug effects
Cell Membrane - drug effects
Cell Membrane - metabolism
Drug Resistance, Fungal
Drug Synergism
Ergosterol - metabolism
Flow cytometry
fluconazole
Fluconazole - pharmacology
Microbial Sensitivity Tests
Microscopy, Electron, Transmission
Mushrooms
Plant Extracts - pharmacology
Protein folding
Rhodamines - metabolism
Rubus
Rubus - chemistry
Rubus Chingii
synergy
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Summary:ABSTRACT This study aimed to investigate the antifungal activity of Rubus chingii extract in combination with fluconazole (FLC) against FLC‐resistant Candida albicans 100 in vitro. A R. chingii extract and FLC‐resistant C. albicans fungus suspension were prepared. The minimum inhibitory concentration and fractional inhibitory concentration index of R. chingii extract combined with FLC against C. albicans were determined, after which growth curves for C. albicans treated with R. chingii extract, FLC alone and a combination of these preparations were constructed. Additionally, the mechanisms of drug combination against C. albicans were explored by flow cytometry, gas chromatographic mass spectrometry and drug efflux pump function detection. R. chingii extract combined with FLC showed significant synergy. Flow cytometry suggested that C. albicans cells mainly arrest in G1 and S phases when they have been treated with the drug combination. The drug combination resulted in a marked decrease in the ergosterol content of the cell membrane. Additionally, efflux of Rhodamine 6G decreased with increasing concentrations of R. chingii extract. R. chingii extract combined with FLC has antifungal activity against FLC‐resistant C. albicans.
ISSN:0385-5600
1348-0421
DOI:10.1111/1348-0421.12357