A Comparison of microCT and microPET for Evaluating Lymph Node Metastasis in a Rat Model

Purpose The demand to optimize multidisciplinary treatment strategies in patients with benign and malignant diseases of the lung and other organs has led to the increased need of mechanistic proof-of-concept studies in preclinical small animal models using new non-invasive imaging methods. Therefore...

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Published in:Molecular imaging and biology 2016-04, Vol.18 (2), p.243-248
Main Authors: Flechsig, Paul, Kratochwil, Clemens, Warth, Arne, Rath, Daniel, Eichwald, Viktoria, Huber, Peter E., Kauczor, Hans-Ulrich, Haberkorn, Uwe, Giesel, Frederik L.
Format: Article
Language:English
Subjects:
Animals
Cell Line, Tumor
Contrast Media
Disease Models, Animal
Humans
Imaging
Lymphatic Metastasis - diagnostic imaging
Male
Medicine
Medicine & Public Health
Positron-Emission Tomography - methods
Radiology
Rats, Nude
Research Article
X-Ray Microtomography - methods
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Summary:Purpose The demand to optimize multidisciplinary treatment strategies in patients with benign and malignant diseases of the lung and other organs has led to the increased need of mechanistic proof-of-concept studies in preclinical small animal models using new non-invasive imaging methods. Therefore, we evaluated the role of microPET and microCT for mediastinal lymph node staging in an orthotopic lung cancer model in rats. Procedures Human lung cancer cells (NCI-H460) were injected transthoracically in nude rats (NIH-RNU). After 2 weeks of tumour growth, animals underwent multiphase contrast-enhanced microCT using ExiTron nano 12000 as a contrast agent and dynamic microPET using the tracer 2-deoxy-2-[ 18 F]fluoro- d -glucose ([ 18 F]FDG). Thereafter, animals were sacrificed for histological analysis. Results Late phase micro X-ray computed tomography (microCT) revealed the best delineation of lymph node metastases, as compared to earlier scans. In terms of an increased [ 18 F]FDG uptake over time, dynamic micro positron emission tomography (microPET) delineated lymph node metastases and enabled metabolic examinations of the induced lung cancer metastases. Conclusion The combination of contrast-enhanced microCT and dynamic microPET is feasible in rats for the visualization of mediastinal lymph node metastases.
ISSN:1536-1632
1860-2002
DOI:10.1007/s11307-015-0890-0