Bacillus cihuensis sp. nov., isolated from rhizosphere soil of a plant in the Cihu area of Taiwan

• Published in: Antonie van Leeuwenhoek 2014-12, Vol.106 (6), p.1147-1155
• Main Authors: Liu, Bo, Liu, Guo-Hong, Sengonca, Cetin, Schumann, Peter, Wang, Ming-Kuang, Tang, Jian-Yang, Chen, Mei-Chun
• Format: Article
• Language: English
• Subjects: Bacillus; Bacillus - classification; Bacillus - genetics; Bacillus - isolation & purification; Bacillus - physiology; Bacillus simplex; Bacterial Typing Techniques; Base Composition; Biomedical and Life Sciences; Cell Wall - chemistry; Cluster Analysis; Cytosol - chemistry; Deoxyribonucleic acid; Diaminopimelic Acid - analysis; DNA; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; DNA, Ribosomal - chemistry; DNA, Ribosomal - genetics; Fatty Acids - analysis; Gram-positive bacteria; Hydrogen-Ion Concentration; Life Sciences; Medical Microbiology; Microbiology; Molecular Sequence Data; Nucleic Acid Hybridization; Original Paper; Peptidoglycan - analysis; Phylogenetics; Phylogeny; Plant Sciences; Quinones - analysis; Rhizosphere; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Sodium chloride; Sodium Chloride - metabolism; Soil Microbiology; Soil microorganisms; Soil Science & Conservation; Taiwan; Taxonomy; Temperature
• ISSN: 0003-6072; 1572-9699
• DOI: 10.1007/s10482-014-0284-3

A Gram-positive, moderately halotolerant, rod-shaped, spore forming bacterium, designated strain FJAT-14515 T was isolated from a soil sample in Cihu area, Taoyuan County, Taiwan. The strain grew at 10–35 °C (optimum at 30 °C), pH 5.7–9.0 (optimum at pH 7.0) and at salinities of 0–5 % (w/v) NaCl (optimum at 1 % w/v). The diagnostic diamino acid of the peptidoglycan of the isolated strain was meso -diaminopimelic acid and major respiratory isoprenoid quinone was MK-7. Major cellular fatty acids were anteiso-C 15:0 (40.6 %), iso-C 15:0 (20.7 %) and the DNA G+C content of strain FJAT-14515 T was 37.1 mol %. A phylogenetic analysis based on 16S rRNA gene sequences indicated that strain FJAT-14515 T belongs to the genus Bacillus , and was most closely related to the reference strains of Bacillus muralis DSM 16288 T (97.6 %) and Bacillus simplex DSM 1321 T (97.5 %). Levels of DNA–DNA relatedness between strain FJAT-14515 T and the reference strains of B. muralis DSM 16288 T and B. simplex DSM 1321 T were 27.9 % ± 3.32 and 44.1 % ± 0.57, respectively. Therefore, on the basis of phenotypic, chemotaxonomic and genotypic properties, strain FJAT-14515 T represents a novel species of the genus Bacillus , for which the name Bacillus cihuensis sp. nov. is proposed. The type strain is FJAT-14515 T (=DSM 25969 T  = CGMCC 1.12697 T ).