Expression analysis of NOS family and HSP genes during thermal stress in goat (Capra hircus)

Approximately 50 genes other than heat shock protein (HSP) expression changes during thermal stress. These genes like nitric oxide synthase (NOS) need proper attention and investigation to find out their possible role in the adaptation to thermal stress in animals. So, the present study was undertak...

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Bibliographic Details
Published in:International journal of biometeorology 2016-03, Vol.60 (3), p.381-389
Main Authors: Yadav, Vijay Pratap, Dangi, Satyaveer Singh, Chouhan, Vikrant Singh, Gupta, Mahesh, Dangi, Saroj K, Singh, Gyanendra, Maurya, Vijay Prakash, Kumar, Puneet, Sarkar, Mihir
Format: Article
Language:English
Subjects:
Animal Physiology
animal stress
Animals
Barbari
bioclimatology
Biological and Medical Physics
Biophysics
Capra hircus
cytoplasm
Earth and Environmental Science
endothelial nitric oxide synthase
Environment
Environmental Health
Female
gene expression
Genes
Goats
Goats - genetics
Goats - physiology
Heat
heat shock proteins
Heat Stress Disorders - genetics
Heat Stress Disorders - metabolism
Heat Stress Disorders - veterinary
homeostasis
HSP70 Heat-Shock Proteins - genetics
HSP70 Heat-Shock Proteins - metabolism
HSP90 Heat-Shock Proteins - genetics
HSP90 Heat-Shock Proteins - metabolism
immunocytochemistry
inducible nitric oxide synthase
Leukocytes, Mononuclear - metabolism
messenger RNA
Meteorology
mononuclear leukocytes
Nitric oxide
Nitric Oxide Synthase - genetics
Nitric Oxide Synthase - metabolism
Original Paper
Plant Physiology
plasma membrane
protein synthesis
quantitative polymerase chain reaction
RNA, Messenger - metabolism
Seasons
Stress
summer
Thermal stress
Western blotting
winter
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Summary:Approximately 50 genes other than heat shock protein (HSP) expression changes during thermal stress. These genes like nitric oxide synthase (NOS) need proper attention and investigation to find out their possible role in the adaptation to thermal stress in animals. So, the present study was undertaken to demonstrate the expressions of inducible form type II NOS (iNOS), endothelial type III NOS (eNOS), constitutively expressed enzyme NOS (cNOS), HSP70, and HSP90 in peripheral blood mononuclear cells (PBMCs) during different seasons in Barbari goats. Real-time polymerase chain reaction, western blot, and immunocytochemistry were applied to investigate messenger RNA (mRNA) expression, protein expression, and immunolocalization of examined factors. The mRNA and protein expressions of iNOS, eNOS, cNOS, HSP70, and HSP90 were significantly higher (P 
ISSN:0020-7128
1432-1254
DOI:10.1007/s00484-015-1035-5