Characterization of Pseudomonas aeruginosa phage KPP21 belonging to family Podoviridae genus N4-like viruses isolated in Japan

ABSTRACT Bacteriophages (phages) belonging to the family Podoviridae genus N4‐like viruses have been used as therapeutic agent in phage therapy against Pseudomonas aeruginosa infections. P. aeruginosa phage KPP21 was isolated in Japan, and phylogenetically investigated the phages belonging to this v...

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Bibliographic Details
Published in:Microbiology and immunology 2016-01, Vol.60 (1), p.64-67
Main Authors: Shigehisa, Ryu, Uchiyama, Jumpei, Kato, Shin-ichiro, Takemura-Uchiyama, Iyo, Yamaguchi, Kotoe, Miyata, Reina, Ujihara, Takako, Sakaguchi, Yoshihiko, Okamoto, Noriaki, Shimakura, Hidekatsu, Daibata, Masanori, Sakaguchi, Masahiro, Matsuzaki, Shigenobu
Format: Article
Language:English
Subjects:
Bacteriology
bacteriophage
Base Composition
Chemical compounds
Chromosome Mapping
DNA polymerase
DNA, Viral - genetics
DNA-directed DNA polymerase
Genes
Genome, Viral
Japan
Microscopy, Electron, Transmission
Molecular Sequence Data
Pathogens
Phage
phage therapy
Phages
Phylogeny
Podoviridae
Podoviridae - classification
Podoviridae - isolation & purification
Podoviridae - ultrastructure
Pseudomonas aeruginosa
Pseudomonas aeruginosa - virology
Pseudomonas Infections - virology
Pseudomonas Phages - classification
Pseudomonas Phages - genetics
Pseudomonas Phages - isolation & purification
Pseudomonas Phages - ultrastructure
Virions
Viruses
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Summary:ABSTRACT Bacteriophages (phages) belonging to the family Podoviridae genus N4‐like viruses have been used as therapeutic agent in phage therapy against Pseudomonas aeruginosa infections. P. aeruginosa phage KPP21 was isolated in Japan, and phylogenetically investigated the phages belonging to this viral genus. Morphological and genetic analyses confirmed that phage KPP21 belongs to the family Podoviridae genus N4‐like viruses. Moreover, phylogenetic analyses based on putative DNA polymerase and major virion protein showed that P. aeruginosa phages belonging to the genus N4‐like viruses are separated into two lineages and that phage KPP21 is in the same clade as phage LUZ7.
ISSN:0385-5600
1348-0421
DOI:10.1111/1348-0421.12347