Phylogeographic analysis of the brown alga Cutleria multifida (Tilopteridales, Phaeophyceae) suggests a complicated introduction history

• Published in: Phycological research 2016, Vol.64 (1), p.3-10
• Main Authors: Kawai, Hiroshi, Kogishi, Keita, Hanyuda, Takeaki, Arai, Shogo, Gurgel, C. Frederico, Nelson, Wendy, Meinesz, Alexandre, Tsiamis, Kostas, Peters, Akira F
• Format: Article
• Language: English
• Subjects: Bacteriology; coasts; cox2; cox3; Cutleria multifida; DNA; females; gametophytes; genes; genetic distance; genetic variation; Haplotypes; introduction; life history; males; marine macroalgae; molecular phylogeny; open reading frames; Phaeophyceae; phylogeography; Tilopteridales
• ISSN: 1322-0829; 1440-1835
• DOI: 10.1111/pre.12113

In depth genetic comparisons of populations of Cutleria multifida (Tilopteridales, Phaeophyceae) collected from Europe, the northwestern Pacific Ocean, Australia and New Zealand using the DNA sequences of four gene regions (the mitochondrial cox2 and cox3 genes, the intergeneric spacer region adjacent to cox3, and the open reading frame) suggested that the northwestern European and Japanese populations were considerably greater in terms of their genetic divergence than Mediterranean, Australian or New Zealand populations. The haplotypes of the populations in northwestern European (distribution range including the type locality, seven haplotypes) and Japanese populations (seven haplotypes) were unique except for one shared haplotype. There were weak but positive correlations between the geographical distance and the genetic divergence among northwestern European and Japanese populations. Moreover, both female and male gametophytes occurred in eight of the nine Japanese localities, suggesting Japanese populations showed normal sexual heteromorphic life history of the species. In light of these results, it appears that Japanese populations were native to the area despite earlier hypothesis. In contrast, Australian and New Zealand populations were composed of only one haplotype that is very close to those found in northwestern Europe and Japan, suggesting a recent introduction history from Europe (or from northeastern Asia via Europe) by ship transport to Australia and New Zealand. The Mediterranean populations included two haplotypes identical to those found in northwestern Europe and Japan, and it is suggestive of transoceanic introductions of some populations between Mediterranean and Japanese coasts.