In vitro activity and killing effect of temporin A on nosocomial isolates of Enterococcus faecalis and interactions with clinically used antibiotics

Objective: To study the in vitro activity of temporin A, a basic, highly hydrophobic, antimicrobial peptide amide derived from the skin of the European red frog Rana temporaria, alone and in combination with co-amoxiclav, imipenem, ciprofloxacin, linezolid and vancomycin, against 42 nosocomial isola...

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Bibliographic Details
Published in:Journal of antimicrobial chemotherapy 2005-02, Vol.55 (2), p.272-274
Main Authors: Giacometti, Andrea, Cirioni, Oscar, Kamysz, Wojciech, D'Amato, Giuseppina, Silvestri, Carmela, Del Prete, Maria Simona, Licci, Alberto, Łukasiak, Jerzy, Scalise, Giorgio
Format: Article
Language:English
Subjects:
Anti-Bacterial Agents - pharmacology
Anti-Bacterial Agents - therapeutic use
antibiotic combinations
Antibiotics. Antiinfectious agents. Antiparasitic agents
antimicrobial peptides
Biological and medical sciences
Cross Infection - drug therapy
Cross Infection - microbiology
Drug Interactions - physiology
enterococci
Enterococcus faecalis
Enterococcus faecalis - drug effects
Enterococcus faecalis - isolation & purification
Gram-positive infections
Humans
Medical sciences
Microbial Sensitivity Tests - statistics & numerical data
Pharmacology. Drug treatments
Proteins - pharmacology
Proteins - therapeutic use
Rana temporaria
susceptibility
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Summary:Objective: To study the in vitro activity of temporin A, a basic, highly hydrophobic, antimicrobial peptide amide derived from the skin of the European red frog Rana temporaria, alone and in combination with co-amoxiclav, imipenem, ciprofloxacin, linezolid and vancomycin, against 42 nosocomial isolates of Enterococcus faecalis. Fourteen of these were resistant to vancomycin. Methods: Antimicrobial activity of temporin A was measured by MIC, MBC and time–kill studies and by the chequerboard titration method. Results: All isolates were inhibited at concentrations of 1 to 16 mg/L. Combination studies carried out with E. faecalis ATCC 29212 and ATCC 51299 demonstrated synergy only when the peptide was combined with co-amoxiclav and imipenem. Conclusions: Our findings show that temporin A is active against E. faecalis and that its activity could be enhanced when it is combined with other antimicrobial agents.
ISSN:0305-7453
1460-2091
DOI:10.1093/jac/dkh545