Risk assessment for developing gliomas: a comparison of two cytogenetic approaches

Chromosome instability (CIN) measured as chromosome aberrations has long been suggested as a cancer susceptibility biomarker. Conventional cytogenetic end-points are now being improved by combining molecular methods, which increases the sensitivity, specificity, and precision of the assay. In this s...

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Published in:Mutation research. Genetic toxicology and environmental mutagenesis 2001-01, Vol.490 (1), p.35-44
Main Authors: El-Zein, Randa, Bondy, Melissa L, Wang, Li-E, de Andrade, Mariza, Sigurdson, Alice J, Bruner, Janet M, Kyritsis, Athanassios P, Levin, Victor A, Wei, Qingyi
Format: Article
Language:English
Subjects:
Biological and medical sciences
Brain tumors
Fluorescence in situ hybridization
Fundamental and applied biological sciences. Psychology
Molecular and cellular biology
Molecular genetics
Mutagenesis. Repair
Peripheral blood lymphocytes
Susceptibility
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Summary:Chromosome instability (CIN) measured as chromosome aberrations has long been suggested as a cancer susceptibility biomarker. Conventional cytogenetic end-points are now being improved by combining molecular methods, which increases the sensitivity, specificity, and precision of the assay. In this study we examined both spontaneous and γ-ray induced CIN in lymphocyte cultures from 51 previously untreated glioma patients and 51 age-, sex- and ethnicity-matched controls. CIN was assessed using two parallel methods: (1) the mutagen sensitivity (MS) assay and (2) the multicolor fluorescence in situ hybridization (FISH) assay. The frequency of spontaneous breaks was significantly higher in glioma patients (mean±S.D., 2.12±1.07) than in controls (1.24±0.86, P
ISSN:1383-5718
1879-3592
DOI:10.1016/S1383-5718(00)00154-6