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Examination of Human Tissue Cytosols for Expression of Sulfotransferase Isoform 1A2 (SULT1A2) Using a SULT1A2-Specific Antibody
Sulfotransferase isoform 1A2 (SULT1A2) is a member of the cytosolic sulfotransferase family of phase II detoxification enzymes. Studies with recombinant enzymes have shown that SULT1A2 can catalyze the bioactivation of several procarcinogens, indicating a potential role in chemical carcinogenesis. H...
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Published in: | Molecular pharmacology 2005-02, Vol.67 (2), p.394-399 |
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container_title | Molecular pharmacology |
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creator | Nowell, Susan Green, Bridgett Tang, Yong Ming Wiese, Rick Kadlubar, Fred F |
description | Sulfotransferase isoform 1A2 (SULT1A2) is a member of the cytosolic sulfotransferase family of phase II detoxification enzymes.
Studies with recombinant enzymes have shown that SULT1A2 can catalyze the bioactivation of several procarcinogens, indicating
a potential role in chemical carcinogenesis. However, previous studies have suggested that the SULT1A2 transcript has a splicing
defect that might prevent it from becoming translated into protein; therefore, we sought to determine the expression of SULT1A2
in tissues. An antibody directed against a region of human SULT1A2 that differs from other known sulfotransferase isoforms
was developed and used to screen a large number of cytosolic fractions from various tissues. Although the SULT1A2 antibody
recognized recombinant SULT1A2 and did not cross-react with other SULT isoforms, the expression of SULT1A2 was not detected
in any tissue examined. These studies suggest that if SULT1A2 is expressed as protein, the levels are very low and that SULT1A2
probably does not play a physiological role in chemical carcinogenesis. |
doi_str_mv | 10.1124/mol.104.006171 |
format | article |
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Studies with recombinant enzymes have shown that SULT1A2 can catalyze the bioactivation of several procarcinogens, indicating
a potential role in chemical carcinogenesis. However, previous studies have suggested that the SULT1A2 transcript has a splicing
defect that might prevent it from becoming translated into protein; therefore, we sought to determine the expression of SULT1A2
in tissues. An antibody directed against a region of human SULT1A2 that differs from other known sulfotransferase isoforms
was developed and used to screen a large number of cytosolic fractions from various tissues. Although the SULT1A2 antibody
recognized recombinant SULT1A2 and did not cross-react with other SULT isoforms, the expression of SULT1A2 was not detected
in any tissue examined. These studies suggest that if SULT1A2 is expressed as protein, the levels are very low and that SULT1A2
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Studies with recombinant enzymes have shown that SULT1A2 can catalyze the bioactivation of several procarcinogens, indicating
a potential role in chemical carcinogenesis. However, previous studies have suggested that the SULT1A2 transcript has a splicing
defect that might prevent it from becoming translated into protein; therefore, we sought to determine the expression of SULT1A2
in tissues. An antibody directed against a region of human SULT1A2 that differs from other known sulfotransferase isoforms
was developed and used to screen a large number of cytosolic fractions from various tissues. Although the SULT1A2 antibody
recognized recombinant SULT1A2 and did not cross-react with other SULT isoforms, the expression of SULT1A2 was not detected
in any tissue examined. These studies suggest that if SULT1A2 is expressed as protein, the levels are very low and that SULT1A2
probably does not play a physiological role in chemical carcinogenesis.</description><subject>Amino Acid Sequence</subject><subject>Antibodies - chemistry</subject><subject>Antibodies - metabolism</subject><subject>Arylsulfotransferase - biosynthesis</subject><subject>Arylsulfotransferase - immunology</subject><subject>Arylsulfotransferase - metabolism</subject><subject>Cytosol - enzymology</subject><subject>Cytosol - immunology</subject><subject>Humans</subject><subject>Immune Sera - chemistry</subject><subject>Immune Sera - metabolism</subject><subject>Isoenzymes - biosynthesis</subject><subject>Isoenzymes - immunology</subject><subject>Isoenzymes - metabolism</subject><subject>Liver - enzymology</subject><subject>Molecular Sequence Data</subject><subject>Protein Array Analysis</subject><subject>Recombinant Proteins - chemistry</subject><subject>Recombinant Proteins - metabolism</subject><issn>0026-895X</issn><issn>1521-0111</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><recordid>eNpFkMFr2zAUh8XoaLOu1x2LLh3bwZmeLFv2MYRsLQR2SAK9CVl-SjRsy5Vs2pz2r9cjhvEO78fje7_DR8gXYEsALn60vlkCE0vGcpDwgSwg45AwALgiC8Z4nhRl9nxDPsX4hzEQWcGuyQ1kGS8EEwvyd_OmW9fpwfmOeksfx1Z3dO9iHJGuz4OPvonU-kA3b33AGGduNzbWD0F30WLQEelT9BPVUlhx-m132O6n8J0eouuOVNP5kOx6NM46Q1fd4Cpfnz-Tj1Y3Ee_mfUsOPzf79WOy_f3rab3aJkZwNiSiqjLNheFMFACywlKzopZojWXcplleyzrXWKJNoS7kNFBUVYla2NLkaNJb8vXS2wf_MmIcVOuiwabRHfoxKpBSCpbKCVxeQBN8jAGt6oNrdTgrYOqfcjUpn7JQF-XTw_3cPFYt1v_x2fEEPFyAkzueXl1A1Z90aLXxjT-eVS4VV2kp0ndunIns</recordid><startdate>20050201</startdate><enddate>20050201</enddate><creator>Nowell, Susan</creator><creator>Green, Bridgett</creator><creator>Tang, Yong Ming</creator><creator>Wiese, Rick</creator><creator>Kadlubar, Fred F</creator><general>American Society for Pharmacology and Experimental Therapeutics</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20050201</creationdate><title>Examination of Human Tissue Cytosols for Expression of Sulfotransferase Isoform 1A2 (SULT1A2) Using a SULT1A2-Specific Antibody</title><author>Nowell, Susan ; Green, Bridgett ; Tang, Yong Ming ; Wiese, Rick ; Kadlubar, Fred F</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c420t-4bb5a24c2048117be9a08d7efcf02f356d7d6ae9ef31d8787818bb9ea4f9c6ec3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Amino Acid Sequence</topic><topic>Antibodies - chemistry</topic><topic>Antibodies - metabolism</topic><topic>Arylsulfotransferase - biosynthesis</topic><topic>Arylsulfotransferase - immunology</topic><topic>Arylsulfotransferase - metabolism</topic><topic>Cytosol - enzymology</topic><topic>Cytosol - immunology</topic><topic>Humans</topic><topic>Immune Sera - chemistry</topic><topic>Immune Sera - metabolism</topic><topic>Isoenzymes - biosynthesis</topic><topic>Isoenzymes - immunology</topic><topic>Isoenzymes - metabolism</topic><topic>Liver - enzymology</topic><topic>Molecular Sequence Data</topic><topic>Protein Array Analysis</topic><topic>Recombinant Proteins - chemistry</topic><topic>Recombinant Proteins - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nowell, Susan</creatorcontrib><creatorcontrib>Green, Bridgett</creatorcontrib><creatorcontrib>Tang, Yong Ming</creatorcontrib><creatorcontrib>Wiese, Rick</creatorcontrib><creatorcontrib>Kadlubar, Fred F</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Molecular pharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nowell, Susan</au><au>Green, Bridgett</au><au>Tang, Yong Ming</au><au>Wiese, Rick</au><au>Kadlubar, Fred F</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Examination of Human Tissue Cytosols for Expression of Sulfotransferase Isoform 1A2 (SULT1A2) Using a SULT1A2-Specific Antibody</atitle><jtitle>Molecular pharmacology</jtitle><addtitle>Mol Pharmacol</addtitle><date>2005-02-01</date><risdate>2005</risdate><volume>67</volume><issue>2</issue><spage>394</spage><epage>399</epage><pages>394-399</pages><issn>0026-895X</issn><eissn>1521-0111</eissn><abstract>Sulfotransferase isoform 1A2 (SULT1A2) is a member of the cytosolic sulfotransferase family of phase II detoxification enzymes.
Studies with recombinant enzymes have shown that SULT1A2 can catalyze the bioactivation of several procarcinogens, indicating
a potential role in chemical carcinogenesis. However, previous studies have suggested that the SULT1A2 transcript has a splicing
defect that might prevent it from becoming translated into protein; therefore, we sought to determine the expression of SULT1A2
in tissues. An antibody directed against a region of human SULT1A2 that differs from other known sulfotransferase isoforms
was developed and used to screen a large number of cytosolic fractions from various tissues. Although the SULT1A2 antibody
recognized recombinant SULT1A2 and did not cross-react with other SULT isoforms, the expression of SULT1A2 was not detected
in any tissue examined. These studies suggest that if SULT1A2 is expressed as protein, the levels are very low and that SULT1A2
probably does not play a physiological role in chemical carcinogenesis.</abstract><cop>United States</cop><pub>American Society for Pharmacology and Experimental Therapeutics</pub><pmid>15528404</pmid><doi>10.1124/mol.104.006171</doi><tpages>6</tpages></addata></record> |
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subjects | Amino Acid Sequence Antibodies - chemistry Antibodies - metabolism Arylsulfotransferase - biosynthesis Arylsulfotransferase - immunology Arylsulfotransferase - metabolism Cytosol - enzymology Cytosol - immunology Humans Immune Sera - chemistry Immune Sera - metabolism Isoenzymes - biosynthesis Isoenzymes - immunology Isoenzymes - metabolism Liver - enzymology Molecular Sequence Data Protein Array Analysis Recombinant Proteins - chemistry Recombinant Proteins - metabolism |
title | Examination of Human Tissue Cytosols for Expression of Sulfotransferase Isoform 1A2 (SULT1A2) Using a SULT1A2-Specific Antibody |
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