Regeneration of Pineapple Plants Via Somatic Embryogenesis and Organogenesis

We have developed efficient methods for plant regeneration, via both embryogenesis and organogenesis, of Smooth Cayenne pineapple, Ananas comosus (L.) Merr. Leaf bases and core (stem) sections of in vitro shoots, produced from culture of crown tip meristem, were used as explants for plant regenerati...

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Bibliographic Details
Published in:In vitro cellular & developmental biology. Plant 2004-01, Vol.40 (1), p.67-74
Main Authors: Firoozabady, E, Moy, Y
Format: Article
Language:English
Subjects:
Ananas comosus
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Summary:We have developed efficient methods for plant regeneration, via both embryogenesis and organogenesis, of Smooth Cayenne pineapple, Ananas comosus (L.) Merr. Leaf bases and core (stem) sections of in vitro shoots, produced from culture of crown tip meristem, were used as explants for plant regeneration as follows: (1) Leaf base and core section explants cultured on Murashige and Skoog (MS) medium containing 41 mu M 4-amino-3,5,6-trichloropicolinic acid (picloram, P) or thidiazuron (T)/P combinations produced embryogenic tissues. Different types of embryogenic tissues (friable emryogenic tissue, embryogenic cell cluster, and chunky embryogenic tissue) have been developed with varying properties in terms of growth rate and state of development. The embryogenic tissues regenerated shoots upon culture on MS medium containing 13 mu M 6-benzylaminopurine (BA) and 1 mu M alpha -naphthaleneacetic acid (NAA) followed by culture on MS medium containing 4 mu M BA. (2) Crown tip meristems cultured on MS medium containing 13 mu M BA followed by leaf explants cultured on MS medium with 27 mu M NAA and 1 mu M BA produced shoots via direct organogenesis. (3) Explants cultured on MS medium containing 5 mu M T and 0.5 mu M indole-3-butyric acid (IBA) produced nodular globular structures, which produced shoots upon culture on MS medium containing 1 mu M BA and 1 mu M gibberellic acid. Shoots obtained from all of the above methods were rooted in half-strength MS medium containing 3 mu M NAA and 2.5 mu M IBA. Plants were transferred to the greenhouse or shipped to Costa Rica for field trials. Somatic embryo-derived plants exhibited 21% spininess, and organogenic-derived plants exhibited 5% spininess in the field trials.
ISSN:1054-5476
DOI:10.1043/1054-5476(2004)040(0067:ROPPVS)2.0.CO;2