Viability and DNA fragmentation of rainbow trout embryos (Oncorhynchus mykiss) obtained from eggs stored at 4 °C

In vitro storage of salmonid eggs leads to aging of the cells causing a decline in quality and reducing their capacity to develop and produce embryos. The quality of salmonid embryos is assessed by morphologic analyses; however, data on the application of biomarkers to determine the cell viability a...

Full description

Saved in:
Bibliographic Details
Published in:Theriogenology 2016-05, Vol.85 (8), p.1499-1506
Main Authors: Ubilla, A., Valdebenito, I., Árias, M.E., Risopatrón, J.
Format: Article
Language:English
Subjects:
Animals
Cold Temperature
DNA
DNA Fragmentation
Embryo
Embryo Culture Techniques - veterinary
Embryo, Nonmammalian - cytology
Embryonic Development
Female
Fertilization in Vitro - veterinary
Genetic Markers
Male
Oncorhynchus mykiss - embryology
Oncorhynchus mykiss - genetics
Oocyte
Ovum - cytology
Ovum - growth & development
Rainbow trout
Storage
Viability
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c301t-2493475124e7e537409033559bffed1178c262a7eebdf2c54364738772985f7b3
cites cdi_FETCH-LOGICAL-c301t-2493475124e7e537409033559bffed1178c262a7eebdf2c54364738772985f7b3
container_end_page 1506
container_issue 8
container_start_page 1499
container_title Theriogenology
container_volume 85
creator Ubilla, A.
Valdebenito, I.
Árias, M.E.
Risopatrón, J.
description In vitro storage of salmonid eggs leads to aging of the cells causing a decline in quality and reducing their capacity to develop and produce embryos. The quality of salmonid embryos is assessed by morphologic analyses; however, data on the application of biomarkers to determine the cell viability and DNA integrity of embryos in these species are limited. The aim of this study was to evaluate the effect on embryo development, viability and DNA fragmentation in the embryonic cells of in vitro storage time at 4 °C of rainbow trout (Oncorhynchus mykiss) eggs. The embryos were obtained by IVF from eggs stored for 0 (control), 48, and 96 hours at 4 °C. At 72 hours after fertilization, dechorionated embryos were examined to determine percentages of developed embryos (embryos with normal cell division morphology), viability (LIVE/DEAD sperm viability kit), and DNA integrity (terminal deoxynucleotidyl transferase [TdT] dUTP nick-end labeling assay). The percentage of developing embryos decreased (P 
doi_str_mv 10.1016/j.theriogenology.2016.01.012
format article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1777979706</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0093691X16000133</els_id><sourcerecordid>1777979706</sourcerecordid><originalsourceid>FETCH-LOGICAL-c301t-2493475124e7e537409033559bffed1178c262a7eebdf2c54364738772985f7b3</originalsourceid><addsrcrecordid>eNqNkM9u1DAQxi0EokvhFZAPHMohi_8kdixxqbYUkCp6AcTNcpxJ1ktiF9sB5W14BJ6hT4arLUjc0Iw00uj3zaf5EHpByZYSKl4dtnkP0YURfJjCuG5Z2W4JLc0eoA1tpao44_Qh2hCieCUU_XKCnqR0IIRwIehjdMJEqzgVYoPiZ2c6N7m8YuN7fPHhHA_RjDP4bLILHocBR-N8F37gHMOSMcxdXEPCZ9fehrhfvd0vCc_rV5fSSxy6XGjoy5UwYxjHhFMOsSxMxvXtz9tfu6fo0WCmBM_u5yn6dPnm4-5ddXX99v3u_KqynNBcsVrxWjaU1SCh4bIminDeNKobBugpla1lghkJ0PUDs03NRS15KyVTbTPIjp-is-Pdmxi-LZCynl2yME3GQ1iSplJKVYqIgr4-ojaGlCIM-ia62cRVU6LvUtcH_W_q-i51TWhpVuTP752Wbob-r_hPzAW4PAJQ_v3uIOpkHXgLvYtgs-6D-z-n3-lMngc</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1777979706</pqid></control><display><type>article</type><title>Viability and DNA fragmentation of rainbow trout embryos (Oncorhynchus mykiss) obtained from eggs stored at 4 °C</title><source>Elsevier</source><creator>Ubilla, A. ; Valdebenito, I. ; Árias, M.E. ; Risopatrón, J.</creator><creatorcontrib>Ubilla, A. ; Valdebenito, I. ; Árias, M.E. ; Risopatrón, J.</creatorcontrib><description>In vitro storage of salmonid eggs leads to aging of the cells causing a decline in quality and reducing their capacity to develop and produce embryos. The quality of salmonid embryos is assessed by morphologic analyses; however, data on the application of biomarkers to determine the cell viability and DNA integrity of embryos in these species are limited. The aim of this study was to evaluate the effect on embryo development, viability and DNA fragmentation in the embryonic cells of in vitro storage time at 4 °C of rainbow trout (Oncorhynchus mykiss) eggs. The embryos were obtained by IVF from eggs stored for 0 (control), 48, and 96 hours at 4 °C. At 72 hours after fertilization, dechorionated embryos were examined to determine percentages of developed embryos (embryos with normal cell division morphology), viability (LIVE/DEAD sperm viability kit), and DNA integrity (terminal deoxynucleotidyl transferase [TdT] dUTP nick-end labeling assay). The percentage of developing embryos decreased (P &lt; 0.05) with storage time of the eggs (95.10 ± 2.55; 88.14 ± 4.50; 79.99 ± 6.60 for 0, 48, and 96 hours, respectively). Similarly, cell viability decreased (P &lt; 0.05; 96.07 ± 7.15; 80.42 ± 8.55; 77.47 ± 7.88 for 0, 48, and 96 hours, respectively), and an increase (P &lt; 0.05) in DNA fragmentation in the embryos was observed at 96-hour storage. A positive correlation was found between cell DNA fragmentation and storage time (r = 0.8173; P &lt; 0.0001). The results revealed that terminal deoxynucleotidyl transferase [TdT] dUTP nick-end labeling assay technique is reliable mean to assess the state of the DNA in salmonid embryos and that in vitro eggs storage for 96h reduces embryo development and cell DNA integrity. DNA integrity evaluation constitutes a biomarker of the quality of the ova and resulting embryos so as to predict their capacity to produce good-quality embryos in salmonids, particularly under culture conditions.</description><identifier>ISSN: 0093-691X</identifier><identifier>EISSN: 1879-3231</identifier><identifier>DOI: 10.1016/j.theriogenology.2016.01.012</identifier><identifier>PMID: 26893166</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Cold Temperature ; DNA ; DNA Fragmentation ; Embryo ; Embryo Culture Techniques - veterinary ; Embryo, Nonmammalian - cytology ; Embryonic Development ; Female ; Fertilization in Vitro - veterinary ; Genetic Markers ; Male ; Oncorhynchus mykiss - embryology ; Oncorhynchus mykiss - genetics ; Oocyte ; Ovum - cytology ; Ovum - growth &amp; development ; Rainbow trout ; Storage ; Viability</subject><ispartof>Theriogenology, 2016-05, Vol.85 (8), p.1499-1506</ispartof><rights>2016 Elsevier Inc.</rights><rights>Copyright © 2016 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c301t-2493475124e7e537409033559bffed1178c262a7eebdf2c54364738772985f7b3</citedby><cites>FETCH-LOGICAL-c301t-2493475124e7e537409033559bffed1178c262a7eebdf2c54364738772985f7b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26893166$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ubilla, A.</creatorcontrib><creatorcontrib>Valdebenito, I.</creatorcontrib><creatorcontrib>Árias, M.E.</creatorcontrib><creatorcontrib>Risopatrón, J.</creatorcontrib><title>Viability and DNA fragmentation of rainbow trout embryos (Oncorhynchus mykiss) obtained from eggs stored at 4 °C</title><title>Theriogenology</title><addtitle>Theriogenology</addtitle><description>In vitro storage of salmonid eggs leads to aging of the cells causing a decline in quality and reducing their capacity to develop and produce embryos. The quality of salmonid embryos is assessed by morphologic analyses; however, data on the application of biomarkers to determine the cell viability and DNA integrity of embryos in these species are limited. The aim of this study was to evaluate the effect on embryo development, viability and DNA fragmentation in the embryonic cells of in vitro storage time at 4 °C of rainbow trout (Oncorhynchus mykiss) eggs. The embryos were obtained by IVF from eggs stored for 0 (control), 48, and 96 hours at 4 °C. At 72 hours after fertilization, dechorionated embryos were examined to determine percentages of developed embryos (embryos with normal cell division morphology), viability (LIVE/DEAD sperm viability kit), and DNA integrity (terminal deoxynucleotidyl transferase [TdT] dUTP nick-end labeling assay). The percentage of developing embryos decreased (P &lt; 0.05) with storage time of the eggs (95.10 ± 2.55; 88.14 ± 4.50; 79.99 ± 6.60 for 0, 48, and 96 hours, respectively). Similarly, cell viability decreased (P &lt; 0.05; 96.07 ± 7.15; 80.42 ± 8.55; 77.47 ± 7.88 for 0, 48, and 96 hours, respectively), and an increase (P &lt; 0.05) in DNA fragmentation in the embryos was observed at 96-hour storage. A positive correlation was found between cell DNA fragmentation and storage time (r = 0.8173; P &lt; 0.0001). The results revealed that terminal deoxynucleotidyl transferase [TdT] dUTP nick-end labeling assay technique is reliable mean to assess the state of the DNA in salmonid embryos and that in vitro eggs storage for 96h reduces embryo development and cell DNA integrity. DNA integrity evaluation constitutes a biomarker of the quality of the ova and resulting embryos so as to predict their capacity to produce good-quality embryos in salmonids, particularly under culture conditions.</description><subject>Animals</subject><subject>Cold Temperature</subject><subject>DNA</subject><subject>DNA Fragmentation</subject><subject>Embryo</subject><subject>Embryo Culture Techniques - veterinary</subject><subject>Embryo, Nonmammalian - cytology</subject><subject>Embryonic Development</subject><subject>Female</subject><subject>Fertilization in Vitro - veterinary</subject><subject>Genetic Markers</subject><subject>Male</subject><subject>Oncorhynchus mykiss - embryology</subject><subject>Oncorhynchus mykiss - genetics</subject><subject>Oocyte</subject><subject>Ovum - cytology</subject><subject>Ovum - growth &amp; development</subject><subject>Rainbow trout</subject><subject>Storage</subject><subject>Viability</subject><issn>0093-691X</issn><issn>1879-3231</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><recordid>eNqNkM9u1DAQxi0EokvhFZAPHMohi_8kdixxqbYUkCp6AcTNcpxJ1ktiF9sB5W14BJ6hT4arLUjc0Iw00uj3zaf5EHpByZYSKl4dtnkP0YURfJjCuG5Z2W4JLc0eoA1tpao44_Qh2hCieCUU_XKCnqR0IIRwIehjdMJEqzgVYoPiZ2c6N7m8YuN7fPHhHA_RjDP4bLILHocBR-N8F37gHMOSMcxdXEPCZ9fehrhfvd0vCc_rV5fSSxy6XGjoy5UwYxjHhFMOsSxMxvXtz9tfu6fo0WCmBM_u5yn6dPnm4-5ddXX99v3u_KqynNBcsVrxWjaU1SCh4bIminDeNKobBugpla1lghkJ0PUDs03NRS15KyVTbTPIjp-is-Pdmxi-LZCynl2yME3GQ1iSplJKVYqIgr4-ojaGlCIM-ia62cRVU6LvUtcH_W_q-i51TWhpVuTP752Wbob-r_hPzAW4PAJQ_v3uIOpkHXgLvYtgs-6D-z-n3-lMngc</recordid><startdate>201605</startdate><enddate>201605</enddate><creator>Ubilla, A.</creator><creator>Valdebenito, I.</creator><creator>Árias, M.E.</creator><creator>Risopatrón, J.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201605</creationdate><title>Viability and DNA fragmentation of rainbow trout embryos (Oncorhynchus mykiss) obtained from eggs stored at 4 °C</title><author>Ubilla, A. ; Valdebenito, I. ; Árias, M.E. ; Risopatrón, J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c301t-2493475124e7e537409033559bffed1178c262a7eebdf2c54364738772985f7b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Animals</topic><topic>Cold Temperature</topic><topic>DNA</topic><topic>DNA Fragmentation</topic><topic>Embryo</topic><topic>Embryo Culture Techniques - veterinary</topic><topic>Embryo, Nonmammalian - cytology</topic><topic>Embryonic Development</topic><topic>Female</topic><topic>Fertilization in Vitro - veterinary</topic><topic>Genetic Markers</topic><topic>Male</topic><topic>Oncorhynchus mykiss - embryology</topic><topic>Oncorhynchus mykiss - genetics</topic><topic>Oocyte</topic><topic>Ovum - cytology</topic><topic>Ovum - growth &amp; development</topic><topic>Rainbow trout</topic><topic>Storage</topic><topic>Viability</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ubilla, A.</creatorcontrib><creatorcontrib>Valdebenito, I.</creatorcontrib><creatorcontrib>Árias, M.E.</creatorcontrib><creatorcontrib>Risopatrón, J.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Theriogenology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ubilla, A.</au><au>Valdebenito, I.</au><au>Árias, M.E.</au><au>Risopatrón, J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Viability and DNA fragmentation of rainbow trout embryos (Oncorhynchus mykiss) obtained from eggs stored at 4 °C</atitle><jtitle>Theriogenology</jtitle><addtitle>Theriogenology</addtitle><date>2016-05</date><risdate>2016</risdate><volume>85</volume><issue>8</issue><spage>1499</spage><epage>1506</epage><pages>1499-1506</pages><issn>0093-691X</issn><eissn>1879-3231</eissn><abstract>In vitro storage of salmonid eggs leads to aging of the cells causing a decline in quality and reducing their capacity to develop and produce embryos. The quality of salmonid embryos is assessed by morphologic analyses; however, data on the application of biomarkers to determine the cell viability and DNA integrity of embryos in these species are limited. The aim of this study was to evaluate the effect on embryo development, viability and DNA fragmentation in the embryonic cells of in vitro storage time at 4 °C of rainbow trout (Oncorhynchus mykiss) eggs. The embryos were obtained by IVF from eggs stored for 0 (control), 48, and 96 hours at 4 °C. At 72 hours after fertilization, dechorionated embryos were examined to determine percentages of developed embryos (embryos with normal cell division morphology), viability (LIVE/DEAD sperm viability kit), and DNA integrity (terminal deoxynucleotidyl transferase [TdT] dUTP nick-end labeling assay). The percentage of developing embryos decreased (P &lt; 0.05) with storage time of the eggs (95.10 ± 2.55; 88.14 ± 4.50; 79.99 ± 6.60 for 0, 48, and 96 hours, respectively). Similarly, cell viability decreased (P &lt; 0.05; 96.07 ± 7.15; 80.42 ± 8.55; 77.47 ± 7.88 for 0, 48, and 96 hours, respectively), and an increase (P &lt; 0.05) in DNA fragmentation in the embryos was observed at 96-hour storage. A positive correlation was found between cell DNA fragmentation and storage time (r = 0.8173; P &lt; 0.0001). The results revealed that terminal deoxynucleotidyl transferase [TdT] dUTP nick-end labeling assay technique is reliable mean to assess the state of the DNA in salmonid embryos and that in vitro eggs storage for 96h reduces embryo development and cell DNA integrity. DNA integrity evaluation constitutes a biomarker of the quality of the ova and resulting embryos so as to predict their capacity to produce good-quality embryos in salmonids, particularly under culture conditions.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>26893166</pmid><doi>10.1016/j.theriogenology.2016.01.012</doi><tpages>8</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0093-691X
ispartof Theriogenology, 2016-05, Vol.85 (8), p.1499-1506
issn 0093-691X
1879-3231
language eng
recordid cdi_proquest_miscellaneous_1777979706
source Elsevier
subjects Animals
Cold Temperature
DNA
DNA Fragmentation
Embryo
Embryo Culture Techniques - veterinary
Embryo, Nonmammalian - cytology
Embryonic Development
Female
Fertilization in Vitro - veterinary
Genetic Markers
Male
Oncorhynchus mykiss - embryology
Oncorhynchus mykiss - genetics
Oocyte
Ovum - cytology
Ovum - growth & development
Rainbow trout
Storage
Viability
title Viability and DNA fragmentation of rainbow trout embryos (Oncorhynchus mykiss) obtained from eggs stored at 4 °C
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-04T20%3A22%3A45IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Viability%20and%20DNA%20fragmentation%20of%20rainbow%20trout%20embryos%20(Oncorhynchus%20mykiss)%20obtained%20from%20eggs%20stored%20at%204%C2%A0%C2%B0C&rft.jtitle=Theriogenology&rft.au=Ubilla,%20A.&rft.date=2016-05&rft.volume=85&rft.issue=8&rft.spage=1499&rft.epage=1506&rft.pages=1499-1506&rft.issn=0093-691X&rft.eissn=1879-3231&rft_id=info:doi/10.1016/j.theriogenology.2016.01.012&rft_dat=%3Cproquest_cross%3E1777979706%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c301t-2493475124e7e537409033559bffed1178c262a7eebdf2c54364738772985f7b3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=1777979706&rft_id=info:pmid/26893166&rfr_iscdi=true