Development of real-time PCR assay for genetic identification of the mottled skate, Beringraja pulchra

Highlights • A real-time PCR was developed to identify Beringraja pulchra. • B. pulchra is served as a fermented fish dish from the Southwestern part of Korea. • The assay demonstrated high specificity suitable for forensic investigation.

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Bibliographic Details
Published in:Forensic science international 2015-10, Vol.255, p.80-84
Main Authors: Hwang, In Kwan, Lee, Hae Young, Kim, Min-Hee, Jo, Hyun-Su, Choi, Dong-Ho, Kang, Pil-Won, Lee, Yang-Han, Cho, Nam-Soo, Park, Ki-Won, Chae, Ho Zoon
Format: Article
Language:English
Subjects:
Animals
Assaying
COI
Cytochrome
Deoxyribonucleic acid
DNA
DNA Primers
DNA Probes
DNA, Mitochondrial - genetics
Electron Transport Complex IV - genetics
Fish
Food
Forensic sciences
Gene sequencing
Genes
Genetics
Markets
Methods
Mitochondrial DNA
Pathology
Polymerase chain reaction
Ray
Real time
Real-Time Polymerase Chain Reaction
Sequence Analysis, DNA
Sequencing
Skates (Fish) - genetics
Species identification
Species Specificity
Taq Polymerase
Thermal cycling
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Description
Summary:Highlights • A real-time PCR was developed to identify Beringraja pulchra. • B. pulchra is served as a fermented fish dish from the Southwestern part of Korea. • The assay demonstrated high specificity suitable for forensic investigation.
ISSN:0379-0738
1872-6283
DOI:10.1016/j.forsciint.2015.05.028