Resonance light scattering detection of fructose bisphosphates using uranyl-salophen complex-modified gold nanoparticles as optical probe

In this paper, we report a resonance light scattering (RLS) method for the determination of fructose bisphosphates (FBPs) in water solution using fructose 1,6-bisphosphate (F-1,6-BP) as a model analyte without the procedure of extracting target analyte. The method used a type of modified gold nanopa...

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Bibliographic Details
Published in:Analytical and bioanalytical chemistry 2015-11, Vol.407 (29), p.8911-8918
Main Authors: Li, Shijun, Liao, Lifu, Wu, Rurong, Yang, Yanyan, Xu, Li, Xiao, Xilin, Nie, Changming
Format: Article
Language:English
Subjects:
acylation
Analytical Chemistry
Biochemistry
Characterization and Evaluation of Materials
Chelation
Chemistry
Chemistry and Materials Science
detection limit
Dynamic Light Scattering
Food Science
Fructose
Fructosediphosphates - analysis
Fructosediphosphates - urine
Gold
Gold - chemistry
Humans
Laboratory Medicine
Ligands
Light
Light scattering
Limit of Detection
Metabolism
Metabolites
Metal Nanoparticles - chemistry
Metal Nanoparticles - ultrastructure
Methods
Monitoring/Environmental Analysis
nanogold
Nanoparticles
Organometallic Compounds - chemistry
phosphates
Research Paper
Resonance
Resonance scattering
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Summary:In this paper, we report a resonance light scattering (RLS) method for the determination of fructose bisphosphates (FBPs) in water solution using fructose 1,6-bisphosphate (F-1,6-BP) as a model analyte without the procedure of extracting target analyte. The method used a type of modified gold nanoparticles (GNPs) as optical probe. The modified GNPs are uranyl-salophen-cysteamine-GNPs (U-Sal-Cy-GNPs) which are obtained through the acylation reaction of carboxylated salophen with cysteamine-capped GNPs (Cy-GNPs) to form Sal-Cy-GNPs and then the chelation reaction of uranyl with tetradentate ligand salophen in the Sal-Cy-GNPs. A FBP molecule is used easily to connect two U-Sal-Cy-GNPs to cause the aggregation of the GNPs by utilizing the specific affinity of uranyl-salophen complex to phosphate group, resulting in the production of strong RLS signal from the system. The amount of FBPs can be determined through detecting the RLS intensity change of the system. A linear range was found to be 2.5 to 75 nmol/L with a detection limit of 0.91 nmol/L under optimal conditions. The method has been successfully used to determine FBPs in real samples with the recoveries of 96.5–103.5 %.
ISSN:1618-2642
1618-2650
DOI:10.1007/s00216-015-9050-2