Urinary metabolites of polycyclic aromatic hydrocarbons in Saudi Arabian schoolchildren in relation to sources of exposure

• Published in: Environmental research 2015-07, Vol.140, p.495-501
• Main Authors: Alghamdi, Mansour A., Alam, Mohammed S., Stark, Christopher, Mohammed, Nuredin, Harrison, Roy M., Shamy, Magdy, Khoder, Mamdouh I., Shabbaj, Ibrahim I., Göen, Thomas
• Format: Article
• Language: English
• Subjects: Adolescent; Adult; Atmosphere; Biomarkers; Biomarkers - urine; Child; Child, Preschool; Consumption; Diet; Environmental Exposure; Exposure; Humans; Male; Mathematical models; Metabolites; Phenanthrene; Polycyclic aromatic hydrocarbons; Polycyclic Aromatic Hydrocarbons - urine; Saudi Arabia; Smoke; Young Adult
• ISSN: 0013-9351; 1096-0953
• DOI: 10.1016/j.envres.2015.04.023

Polycyclic aromatic hydrocarbons contain a number of known carcinogenic compounds, and urinary biomarkers have been widely used as a measure of exposure but quantitative relationships with exposure variables have proved elusive. This study aimed to quantify the relationship between exposures to phenanthrene and pyrene from atmospheric and dietary sources with the excretion of 1-hydroxypyrene and hydroxyphenanthrenes in urine as biomarkers of exposure. The study population consisted of 204 male schoolchildren attending three schools in different parts of Jeddah, Saudi Arabia who provided urine samples on each of three consecutive days. Outdoor air measurements of polycyclic aromatic hydrocarbons were made at the schools and the children provided information on diet, exposure to environmental tobacco smoke and incense, and various lifestyle factors through a questionnaire. Mixed models with random effects for subjects nested within site were fitted in order to examine the relationship between exposure variables and urinary PAH metabolites. A unit increase (1ngm−3) in ambient pyrene (particulate plus gaseous phase) was associated with a 3.5% (95% CI: 1.01%, 5.13%) increase in urinary 1-hydroxypyrene concentration. A unit increase in ambient phenanthrene was associated with a 1.01% (95% CI: 0.03%, 2.02%) increase in total hydroxyphenanthrene concentrations. Consumption of chargrilled food increased the 1-hydroxypyrene and hydroxyphenanthrene concentrations by 24% (95% CI: 11%, 37%) and 17% (95% CI: 8%, 26%) respectively. We did not find evidence of association for environmental tobacco smoke exposure or incense burning. It is concluded that both respiratory exposure and consumption of chargrilled food are considerable sources of PAH exposure in this population as reflected by concentrations of urinary biomarkers. •1-hydroxypyrene and hydroxyphenanthrenes measured in urine of 204 schoolchildren.•Children from different parts of city show statistically different concentrations.•Elevated hydroxy-PAH associated with atmospheric and dietary exposures.•Exposure-biomarker relationships are quantified for air and diet.