Fluorescence enhancement and multiple protein detection in ZnO nanostructure microfluidic devices

• Published in: Biosensors & bioelectronics 2016-01, Vol.75, p.285-292
• Main Authors: Sang, Chen-Hsiang, Chou, Shu-Jen, Pan, F.M., Sheu, Jeng-Tzong
• Format: Article
• Language: English
• Subjects: Animals; Biosensing Techniques; Biotin - chemistry; Channels; Crossovers; Dyes; Fluorescence; Fluorescence enhancement; Immunoglobulin G - isolation & purification; Lab-On-A-Chip Devices; Mice; Microfluidic channel; Microfluidics; Multiple protein detection; Nanostructure; Nanostructures; Nanowires - chemistry; Proteins; Rabbits; Zinc oxide; Zinc Oxide - chemistry; ZnO nanostructures
• ISSN: 0956-5663; 1873-4235
• DOI: 10.1016/j.bios.2015.08.050

In this study, different morphological ZnO nanostructures, those of sharp nanowires (NWs), rod NWs, and hexahedral-puncheon nanostructures, were grown in microfluidic channels on the same glass substrate. Characterizations of correspondent biomolecule binding properties were simulated and demonstrated. The surface was modified using 3-ammineopropyl-triethoxysilane (3-APTES) and biotin-N-hydroxysuccinimide ester (NHS-biotin). Different concentrations (4.17pM to 41.7nM) of dye-conjugated streptavidin were simultaneously infused through the second microfluidic channels, which lie 90° from the first microfluidic channels. The florescent intensity at the crossover areas showed good agreement with simulations, with sharp ZnO NWs exhibiting the largest dynamic range and the highest fluorescent intensity. We further characterize correspondent protein detection using sharp ZnO NWs. The surfaces of these ZnO NWs were modified with mouse immunoglobulin G (IgG), infused through the second microfluidic channels with dye-conjugated (Alexa 546) anti-mouse IgG in different concentrations. Concentrations ranging from 417fM to 41.7nM can be resolved using sharp ZnO NWs. Finally, multiple protein detection was demonstrated using a five-by-eight microfluidic channel array. Fluorescence images present clear multiple detections at the crossover areas when using the sharp ZnO NWs for simultaneous dye-conjugated anti-mouse IgG and dye-conjugated anti-rabbit IgG (Alexa 647) detection. •Different morphological ZnO nanostructures were grown in microfluidic channels.•The sharp ZnO NWs enhance the fluorescent intensity and exhibit the largest dynamic range.•A simultaneous multiple protein detection was demonstrated on the same microfluidic device.