Determination of endocrine disrupting compounds and their metabolites in fish bile

This work describes a new methodology for the simultaneous determination of a large variety of emerging and persistent organic compounds and some of their metabolites in fish bile samples. The target compounds were musk fragrances, alkyl phenols, hormones, pesticides, phthalate esters and bisphenol-...

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Bibliographic Details
Published in:The Science of the total environment 2015-12, Vol.536, p.261-267
Main Authors: Ros, Oihana, Izaguirre, Jon Kepa, Olivares, Maitane, Bizarro, Cristina, Ortiz-Zarragoitia, Maren, Cajaraville, Miren Pilar, Etxebarria, Nestor, Prieto, Ailette, Vallejo, Asier
Format: Article
Language:English
Subjects:
Analytical chemistry
Animals
Benzhydryl Compounds - analysis
Benzhydryl Compounds - metabolism
Bile - chemistry
Bile - metabolism
Bioconcentration factor
Bisphenol A
Cartridges
Chelon labrosus
Disrupting
Endocrine disrupting compounds
Endocrine Disruptors - analysis
Endocrine Disruptors - metabolism
Environmental Monitoring
Fish
Fish bile
Fishes - metabolism
Freshwater
Gas Chromatography-Mass Spectrometry
Limit of Detection
Metabolites
Musk
Phenols - analysis
Phenols - metabolism
Phthalic Acids - analysis
Phthalic Acids - metabolism
Plexa
Sampling
Smegmamorpha
Solid Phase Extraction
Water Pollutants, Chemical - analysis
Water Pollutants, Chemical - metabolism
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Summary:This work describes a new methodology for the simultaneous determination of a large variety of emerging and persistent organic compounds and some of their metabolites in fish bile samples. The target compounds were musk fragrances, alkyl phenols, hormones, pesticides, phthalate esters and bisphenol-A, all of them with a known endocrine disrupting effect. To achieve the determination these three steps were optimized: i) an enzymatic hydrolysis of the metabolites to render the unconjugated compounds; ii) the solid phase extraction of the target analytes (Plexa cartridges 200-mg); and, iii) a clean-up of the extracts (Florisil cartridges 1-g). The samples were analyzed by gas-chromatography–mass spectrometry (GC–MS), though the polar fraction required a previous derivatization with O-bis (trimethylsilyl) trifluoroacetamide. Good apparent recoveries (63–122%), repeatability (
ISSN:0048-9697
1879-1026
DOI:10.1016/j.scitotenv.2015.07.074