Determining sequences and post-translational modifications of novel conotoxins in Conus victoriae using cDNA sequencing and mass spectrometry

• Published in: Journal of mass spectrometry. 2004-05, Vol.39 (5), p.548-557
• Main Authors: Jakubowski, Jennifer A., Keays, David A., Kelley, Wayne P., Sandall, David W., Bingham, Jon-Paul, Livett, Bruce G., Gayler, Ken R., Sweedler, Jonathan V.
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Aminoacids, peptides. Hormones. Neuropeptides; Analytical, structural and metabolic biochemistry; Animal poisons toxicology. Antivenoms; Animals; Base Sequence; Biological and medical sciences; cDNA sequencing; Cloning, Molecular; conotoxin; Conotoxins - chemistry; Conotoxins - genetics; Conotoxins - metabolism; Conus victoriae; DNA, Complementary - genetics; electrospray ionization mass spectrometry; Fundamental and applied biological sciences. Psychology; Mass Spectrometry; matrix-assisted laser desorption/ionization mass spectrometry; Medical sciences; Molecular Sequence Data; Mollusk Venoms - chemistry; Mollusk Venoms - genetics; post-translational modification; Protein Processing, Post-Translational; Proteins; Sequence Analysis, DNA; Sequence Homology, Amino Acid; Toxicology
• ISSN: 1076-5174; 1096-9888
• DOI: 10.1002/jms.624

A combination of cDNA cloning and detailed mass spectrometric analyses was employed to identify novel conotoxins from Conus victoriae. Eleven conotoxin sequences were determined using molecular methods: one belonging to the A superfamily (Vc1.1), six belonging to the O superfamily (Vc6.1–Vc6.6) and four members of the T superfamily (Vc5.1–Vc5.4). In order to verify the sequences and identify the post‐translational modifications (excluding the disulfide connectivity) of three Conus victoriae conotoxins, vc1a, vc5a and vc6a, deduced from sequences Vc1.1, Vc5.1, and Vc6.1, respectively, liquid chromatography/electrospray ionization ion trap mass spectrometry, matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry and nanospray ionization ion trap mass spectrometry with collisionally induced dissociation were performed on reduced and alkylated venom fractions. We report that vc1a, the native form of α‐conotoxin Vc1.1 (an unmodified 16 amino acid residue peptide that has notable pain‐relieving capabilities), includes a hydroxyproline and a γ‐carboxyglutamate residue. Conotoxin vc5a is a 10‐residue peptide with two disulfide bonds and a hydroxyproline and vc6a is a 25 amino acid peptide with three disulfide bonds. Copyright © 2004 John Wiley & Sons, Ltd.