Evidence for marine origin and microbial-viral habitability of sub-zero hypersaline aqueous inclusions within permafrost near Barrow, Alaska

• Published in: FEMS microbiology ecology 2016-05, Vol.92 (5), p.fiw053-fiw053
• Main Authors: Colangelo-Lillis, J., Eicken, H., Carpenter, S. D., Deming, J. W.
• Format: Article
• Language: English
• Subjects: Alaska; Bacteria - classification; Bacteria - isolation & purification; Environmental aspects; Frozen ground; Isotopes; Microorganisms; Permafrost - chemistry; Permafrost - microbiology; Permafrost - virology; Physiological aspects; Salinity; Seawater - chemistry; Seawater - microbiology; Seawater - virology; Temperature; Viruses - classification; Viruses - isolation & purification; Water - chemistry
• ISSN: 1574-6941; 0168-6496; 1574-6941
• DOI: 10.1093/femsec/fiw053

Cryopegs are sub-surface hypersaline brines at sub-zero temperatures within permafrost; their global extent and distribution are unknown. The permafrost barrier to surface and groundwater advection maintains these brines as semi-isolated systems over geological time. A cryopeg 7 m below ground near Barrow, Alaska, was sampled for geochemical and microbiological analysis. Sub-surface brines (in situ temperature of –6 °C, salinity of 115 ppt), and an associated sediment-infused ice wedge (melt salinity of 0.04 ppt) were sampled using sterile technique. Major ionic concentrations in the brine corresponded more closely to other (Siberian) cryopegs than to Standard seawater or the ice wedge. Ionic ratios and stable isotope analysis of water conformed to a marine or brackish origin with subsequent Rayleigh fractionation. The brine contained ∼1000× more bacteria than surrounding ice, relatively high viral numbers suggestive of infection and reproduction, and an unusually high ratio of particulate to dissolved extracellular polysaccharide substances. A viral metagenome indicated a high frequency of temperate viruses and limited viral diversity compared to surface environments, with closest similarity to low water activity environments. Interpretations of the results underscore the isolation of these underexplored microbial ecosystems from past and present oceans. Measurements made from liquid brine within Arctic permafrost indicate an ancient marine origin, active viral–microbe dynamics and large amounts of extracellular polysaccharide substances. Graphical Abstract Figure. Measurements made from liquid brine within Arctic permafrost indicate an ancient marine origin, active viral–microbe dynamics and large amounts of extracellular polysaccharide substances.