In vivo monitoring of macrophage infiltration in experimental autoimmune neuritis by magnetic resonance imaging

We report on the in vivo assessment of macrophage infiltration in adoptive transfer experimental autoimmune neuritis (AT-EAN), a prototypic Th-1-mediated autoimmune disorder of the peripheral nervous system (PNS). Lewis rats received systemic injections of superparamagnetic iron oxide (SPIO) particl...

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Bibliographic Details
Published in:Journal of neuroimmunology 2004-04, Vol.149 (1), p.142-146
Main Authors: Stoll, Guido, Wesemeier, Carmen, Gold, Ralf, Solymosi, Laszlo, Toyka, Klaus V., Bendszus, Martin
Format: Article
Language:English
Subjects:
Animals
Autoimmunity
Cauda Equina - pathology
CD4-Positive T-Lymphocytes - metabolism
Cell Movement
EAN
Ectodysplasins
Encephalomyelitis, Autoimmune, Experimental - chemically induced
Encephalomyelitis, Autoimmune, Experimental - pathology
Female
Ferric Compounds
Ferrosoferric Oxide
Immunohistochemistry - methods
Iron - metabolism
Macrophage
Macrophages - pathology
Magnetic resonance imaging
Magnetic Resonance Imaging - methods
Membrane Proteins - metabolism
Rats
Rats, Inbred Lew
SPIO
Time Factors
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Summary:We report on the in vivo assessment of macrophage infiltration in adoptive transfer experimental autoimmune neuritis (AT-EAN), a prototypic Th-1-mediated autoimmune disorder of the peripheral nervous system (PNS). Lewis rats received systemic injections of superparamagnetic iron oxide (SPIO) particles at days 2, 3, 4, 5, or 9 after adoptive transfer of P2-specific T-cells and were scanned by standard 1.5 T magnetic resonance imaging (MRI) always 24 h later. MRI revealed focal signal loss of the cauda equina indicating iron accumulation in spinal nerves already at the preclinical stage (day 3). Signal loss peaked at day 4, when first clinical signs developed. At the maximum of clinical disease, signal loss already declined and disappeared on days 6 and 10. In spinal nerve sections, Perl's iron stain showed focal cellular accumulation of SPIO at days 3, 4, and 5 in ED1-positive macrophages. Spinal nerves at days 6 and 10 exhibited massive macrophage infiltrates, but no more iron deposition. In conclusion, SPIO-enhanced MRI provides a novel in vivo tool to assess the timing of macrophage entry into target tissues during an immunopathologic attack and holds promise to monitor immunotherapeutic interventions.
ISSN:0165-5728
1872-8421
DOI:10.1016/j.jneuroim.2003.12.024