Epidermal thymic stromal lymphopoietin predicts the development of atopic dermatitis during infancy

• Published in: Journal of allergy and clinical immunology 2016-04, Vol.137 (4), p.1282-1285.e4
• Main Authors: Kim, Jihyun, MD, PhD, Kim, Byung Eui, MD, PhD, Lee, Jeongok, PhD, Han, Youngshin, PhD, Jun, Hyun-Young, MS, Kim, Hyunmi, MS, Choi, Jaehee, MD, Leung, Donald Y.M., MD, PhD, Ahn, Kangmo, MD, PhD
• Format: Article
• Language: English
• Subjects: Age; Allergies; Allergy and Immunology; Asthma; Biomarkers; Biomarkers - metabolism; Birth weight; Breastfeeding & lactation; Case-Control Studies; Child, Preschool; Cytokines - metabolism; Dermatitis; Dermatitis, Atopic - diagnosis; Dermatitis, Atopic - metabolism; Education; Epidermis - metabolism; Family medical history; Fatty acids; Female; Health risk assessment; Humans; Infant; Inflammation; Logistic Models; Logistics; Male; Mass spectrometry; Parent educational background; Pregnancy; Prospective Studies; Proteins; Scientific imaging
• ISSN: 0091-6749; 1097-6825
• DOI: 10.1016/j.jaci.2015.12.1306

Mass spectrometry was carried out as previously described.E1 Samples were run in triplicate on an Agilent 1200 series HPLC (Agilent Technologies, Santa Clara, Calif) and Agilent ETD ion trap (model 6340) mass spectrometer with an HPLC chip to evaluate the expression of filaggrin, alpha enolase, corneodesmosin, fatty acid-binding protein, serpin B3, transglutaminase 3, and TSLP. In a univariable analysis, candidate variables for adjustment included epidermal protein levels, sex, parental history of allergic diseases, delivery mode, maternal education levels, move to a new house during pregnancy, mold exposure during pregnancy, exclusive breast-feeding during the first 4 months of life, and TEWL at age 2 months.