Microcrystal delivery by pulsed liquid droplet for serial femtosecond crystallography

A liquid‐droplet injector has been developed that delivers pristine microcrystals to an X‐ray irradiation area for conducting serial femtosecond crystallography (SFX) with an X‐ray free‐electron laser (XFEL). By finely tuning the pulsed liquid droplets in time and space, a high hit rate of the XFEL...

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Bibliographic Details
Published in:Acta crystallographica. Section D, Structural biology Structural biology, 2016-04, Vol.72 (4), p.520-523
Main Authors: Mafuné, Fumitaka, Miyajima, Ken, Tono, Kensuke, Takeda, Yoshihiro, Kohno, Jun-ya, Miyauchi, Naoya, Kobayashi, Jun, Joti, Yasumasa, Nango, Eriko, Iwata, So, Yabashi, Makina
Format: Article
Language:English
Subjects:
Crystallography, X-Ray - instrumentation
Crystallography, X-Ray - methods
liquid-droplet injector
lysozyme
serial femtosecond crystallography
X-ray free-electron laser
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Summary:A liquid‐droplet injector has been developed that delivers pristine microcrystals to an X‐ray irradiation area for conducting serial femtosecond crystallography (SFX) with an X‐ray free‐electron laser (XFEL). By finely tuning the pulsed liquid droplets in time and space, a high hit rate of the XFEL pulses to microcrystals in the droplets was achieved for measurements using 5 µm tetragonal lysozyme crystals, which produced 4265 indexable diffraction images in about 30 min. The structure was determined at a resolution of 2.3 Å from
ISSN:2059-7983
2059-7983
DOI:10.1107/S2059798316001480