An In-Gel Assay of a Recombinant Western Corn Rootworm (Diabrotica virgifera virgifera) Cysteine Proteinase Expressed in Yeast

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Bibliographic Details
Published in:Analytical biochemistry 2000-06, Vol.282 (1), p.153-155
Main Authors: Koiwa, Hisashi, Paino D'Urzo, Matilde, Zhu-Salzman, Keyan, Ibeas, Jose Ignacio, Shade, Richard E., Murdock, Larry L., Bressan, Ray A., Hasegawa, Paul M.
Format: Article
Language:English
Subjects:
Animals
Chrysomelidae
Cockroaches - enzymology
Cystatins - pharmacology
Cysteine Endopeptidases - chemistry
Cysteine Endopeptidases - genetics
Cysteine Endopeptidases - isolation & purification
Cysteine Proteinase Inhibitors - analysis
Diabrotica virgifera
Enzyme Precursors - analysis
Mutagenesis
Pichia - enzymology
Pichia - metabolism
Protein Isoforms
proteinase
Recombinant Proteins - analysis
Saccharomyces cerevisiae - enzymology
Saccharomyces cerevisiae - metabolism
Soybean Proteins
Transcription, Genetic
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ISSN:0003-2697
1096-0309
DOI:10.1006/abio.2000.4591