Enforced Bcl-2 Expression Overrides Serum and Feeder Cell Requirements for Mouse Embryonic Stem Cell Self-Renewal

Leukemia inhibitory factor (LIF) is required, but not sufficient, for pluripotent mouse embryonic stem (ES) cell expansion in vitro in the absence of serum or a feeder cell layer, suggesting that additional signals are provided by serum or feeders that are necessary to support self-renewal. Here we...

Full description

Saved in:
Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 2005-03, Vol.102 (9), p.3312-3317
Main Authors: Yamane, Toshiyuki, Dylla, Scott J., Muijtjens, Manja, Weissman, Irving L.
Format: Article
Language:English
Subjects:
Animals
Apoptosis - physiology
Base Sequence
Biological Sciences
Blood
Bone Morphogenetic Proteins - metabolism
Cell culture
Cell culture techniques
Cell Division
Cell growth
Cell lines
Cellular differentiation
Chimera
Cultured cells
DNA Primers
Embryo, Mammalian - cytology
Embryos
Feeder cells
Female
Flow Cytometry
Humans
Immunohistochemistry
Mice
Mice, Inbred C57BL
Neurons
Pluripotent stem cells
Polymerase Chain Reaction
Proteins
Proto-Oncogene Proteins c-bcl-2 - metabolism
Proto-Oncogene Proteins c-bcl-2 - physiology
Rodents
Stem cells
Stem Cells - cytology
Stromal cells
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Leukemia inhibitory factor (LIF) is required, but not sufficient, for pluripotent mouse embryonic stem (ES) cell expansion in vitro in the absence of serum or a feeder cell layer, suggesting that additional signals are provided by serum or feeders that are necessary to support self-renewal. Here we show that transgenic ES cell lines expressing Bcl-2, an antiapoptotic protein, continue to self-renew in serum- and feeder-free conditions when supplemented with LIF; even in the absence of bone morphogenic proteins. Bcl-2-expressing clones sustain the characteristics of undifferentiated, pluripotent ES cells during long-term culture, and maintain their potential to differentiate into mature cell types. These results suggest that LIF and Bcl-2 overexpression are sufficient to expand these mouse pluripotent stem cells in vitro.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.0500167102