Peculiarities of Gene Transfer into Mesenchymal Stem Cells

Murine mesenchymal stem cells in long-term bone marrow culture were genetically labeled using lentiviral vector carrying enhanced green fluorescent protein (eGFP) reporter gene under SFFV promoter or without it. We studied the developmental fate of labeled mesenchymal stem cells in stromal cell laye...

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Bibliographic Details
Published in:Bulletin of experimental biology and medicine 2015-05, Vol.159 (1), p.134-137
Main Authors: Sats, N. V., Shipunova, I. N., Bigil’diev, A. E., Kostyushev, D. S., Drize, N. I.
Format: Article
Language:English
Subjects:
Analysis
Animals
Biomedical and Life Sciences
Biomedicine
Bone Marrow Cells - cytology
Cell Biology
Cells, Cultured
Fibroblasts - cytology
Fluorescence
Gene therapy
Gene Transfer Techniques
Genes
Genes, Reporter - genetics
Genetic Vectors - genetics
Green Fluorescent Proteins - genetics
Internal Medicine
Laboratory Medicine
Lentivirus
Mesenchymal Stem Cell Transplantation
Mesenchymal Stromal Cells - cytology
Mice
Mice, Inbred C57BL
Mice, Inbred CBA
Pathology
Promoter Regions, Genetic
Stem cells
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Summary:Murine mesenchymal stem cells in long-term bone marrow culture were genetically labeled using lentiviral vector carrying enhanced green fluorescent protein (eGFP) reporter gene under SFFV promoter or without it. We studied the developmental fate of labeled mesenchymal stem cells in stromal cell layers of long-term bone marrow culture and in ectopic hemopoietic foci formed by these stromal layers under the renal capsule of syngeneic mice. The frequency of labeled polypotent stromal precursors (fibroblast CFU) was analyzed in adherent cell layers of long-term culture and ectopic foci formed from them. The proportion of labeled fibroblast CFU in ectopic foci increased by 10 times in case of implantation of adherent cell layers infected with lentivirus containing eGFP reporter gene without promoter. eGFP expression leads to rejection of labeled stromal cells. Labeling with eGFP-carrying vector without promoter makes possible long-term tracking of mesenchymal stromal cells.
ISSN:0007-4888
1573-8221
DOI:10.1007/s10517-015-2908-7