Quantitative Morphochemical Characterization of the Neurons in Substantia Nigra of Rat Brain and Its Volume Reconstruction

Three cell compartments differing by size and proportion of neurons were identifi ed by 3D reconstruction of the substantia nigra pars compacta of the rat brain based on immunohistochemical localization of tyrosine hydroxylase, a marker of dopamine neurons. Dopaminepositive neurons prevailed over do...

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Bibliographic Details
Published in:Bulletin of experimental biology and medicine 2014-04, Vol.156 (6), p.861-864
Main Authors: Khudoerkov, R. M., Voronkov, D. N., Dikalova, Yu. V.
Format: Article
Language:English
Subjects:
Analysis of Variance
Animals
Biomedical and Life Sciences
Biomedicine
Brain
Cell Biology
Chemical properties
Dopaminergic Neurons - chemistry
Dopaminergic Neurons - cytology
Imaging, Three-Dimensional
Immunohistochemistry
Internal Medicine
Laboratory Medicine
Male
Morphology and Pathomorphology
Neurons
Pathology
Rats
Rats, Wistar
Substantia Nigra - cytology
Tyrosine 3-Monooxygenase
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Summary:Three cell compartments differing by size and proportion of neurons were identifi ed by 3D reconstruction of the substantia nigra pars compacta of the rat brain based on immunohistochemical localization of tyrosine hydroxylase, a marker of dopamine neurons. Dopaminepositive neurons prevailed over dopamine-free neurons (1.45:1) in the most voluminous (75 %) dorsal part, and in smaller lateral and ventral parts, inverse cell ratios were observed: 0.54:1 and 0.78:1, respectively. Morphometry characterized the substantia nigra pars compacta as a structure consisting not only of several parts, but of horizons and showed differences between the neurons both in several parts and in several layers within the part. The revealed morphochemical heterogeneity of the substantia nigra pars compacta provides better understanding of the selective damage to its structures in Parkinson’s disease.
ISSN:0007-4888
1573-8221
DOI:10.1007/s10517-014-2470-8