Effects of Combined Treatment with Complex S. typhimurium Antigens and Factors Stimulating Osteogenesis (Curettage, BMP-2) on Multipotent Bone Marrow Stromal Cells and Serum Concentration of Cytokines in CBA Mice

• Published in: Bulletin of experimental biology and medicine 2015-02, Vol.158 (4), p.465-470
• Main Authors: Gorskaya, Yu. F., Danilova, T. A., Karyagina, A. S., Lunin, V. G., Grabko, V. I., Bartov, M. S., Gromov, A. V., Grunina, T. M., Soboleva, L. A., Shapoval, I. M., Nesterenko, V. G.
• Format: Article
• Language: English
• Subjects: Analysis; Animals; Antigens; Antigens, Bacterial - pharmacology; Biomedical and Life Sciences; Biomedicine; Bone Marrow Cells - drug effects; Bone Morphogenetic Protein 2 - pharmacology; Cell Biology; Curettage; Cytokines; Cytokines - blood; Interferon-gamma - blood; Interleukin-10 - blood; Interleukin-2 - blood; Internal Medicine; Laboratory Medicine; Mice; Mice, Inbred CBA; Osteogenesis - drug effects; Pathology; Phosphatases; Salmonella typhimurium; Salmonella typhimurium - immunology; Stromal Cells - drug effects; Tumor Necrosis Factor-alpha - blood
• ISSN: 0007-4888; 1573-8221
• DOI: 10.1007/s10517-015-2786-z

The content of multipotent stromal cells (MSC) in the bone marrow and efficiency of their cloning (ECF-MSC) increased by 3 times 1 day after administration of complex S. typhimurium antigens to CBA mice, while the relative content of alkaline phosphatase-positive MSC colonies (marker of osteogenesis; P + colonies) decreased from 14% (control) to 3%. After administration of the complex S. typhimurium antigens to CBA mice 3 h after (or 3 h before) curettage or treatment with morphogenetic protein (BMP-2), the content of MSC and ECF-MSC decreased on the next day by ~3 times in comparison with animals receiving antigens alone and approached the control level. The relative content of P + colonies increased to 20 and 35%, respectively, in comparison with animals receiving antigens (3%), but was signifi cantly lower than after curettage (34%) or BMP-2 (42%) administration. Expression of IL-1β, IL-6, IL-12, TNF-α, and IFN-γ genes in the primary cultures of stromal bone marrow cells induced by antigen administration was suppressed, while the concentrations of IL-12 and TNF-α in the culture medium sharply decreased after antigen treatment in combination with curettage or BMP-2 administration. Administration of complex S. typhimurium antigens after pretreatment with BMP-2 (3 h before) was associated with a decrease in serum levels of IL-2, IFN-γ, IL-12, and TNF-α in mice receiving BMP-2+ S. typhimurium group 4 h after treatment in comparison with the animals receiving only S. typhimurium antigens alone by 1.9, 4.4, 1.5, and 6 times, respectively, i.e. to normal level or below it, while the concentration of IL-10 increased by almost 2 times, which probably reflected anti-inflammatory properties of BMP-2. These data probably attest to competitive relations between osteogenesis and immune response at the level of MSC.