Development of Rat Embryonic Spinal Ganglion Cells in Damaged Nerve

The development of dissociated cells from rat embryonic spinal ganglion after transplantation to damaged nerve of adult animals was studied using immunohistochemical differentiation markers of neural and glial cells. The cell suspension obtained after dissociation of rat embryonic spinal ganglia (em...

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Bibliographic Details
Published in:Bulletin of experimental biology and medicine 2014-09, Vol.157 (5), p.637-640
Main Authors: Petrova, E. S., Isaeva, E. N., Korzhevskii, D. E.
Format: Article
Language:English
Subjects:
Animals
Biomedical and Life Sciences
Biomedicine
Cell Biology
Embryonic development
Ganglia, Spinal - cytology
Ganglia, Spinal - embryology
Ganglia, Spinal - injuries
Ganglion
Internal Medicine
Laboratory Medicine
Morphology and Pathomorphology
Pathology
Rats
Rats, Wistar
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Summary:The development of dissociated cells from rat embryonic spinal ganglion after transplantation to damaged nerve of adult animals was studied using immunohistochemical differentiation markers of neural and glial cells. The cell suspension obtained after dissociation of rat embryonic spinal ganglia (embryonic day 15) was injected into the proximal segment of crushed sciatic nerve. The nerve was damaged by ligation for 40 sec. Progenitor cells were labeled with 5-bromo-2′-deoxyuridine (BrdU) before transplantation. BrdU-immunopositive cells were detected in the nerve trunks of recipients on days 1, 21, and 28 after transplantation. Dissociated cells of rat embryonic spinal ganglion (embryonic day 15) survived for at least 4 weeks after transplantation to the nerve and differentiate into NeuN-immunopositive neurons with morphological properties of sensory neurons and satellite cells containing S100 protein.
ISSN:0007-4888
1573-8221
DOI:10.1007/s10517-014-2633-7