Effect of Platelet Releasate on Osteogenic Differentiation of Human Mesenchymal Bone Marrow Stem Cells

We studied the effect of platelet releasate on osteogenic differentiation of human mesenchymal BM stem cells. Histological staining and reverse transcription PCR analysis showed that addition of platelet releasate to osteogenic differentiation medium stimulated the formation of calcium ossificates a...

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Bibliographic Details
Published in:Bulletin of experimental biology and medicine 2014-02, Vol.156 (4), p.560-565
Main Authors: Kosmacheva, S. M., Danilkovich, N. N., Shchepen’, A. V., Ignatenko, S. I., Potapnev, M. P.
Format: Article
Language:English
Subjects:
Alkaline Phosphatase - genetics
Alkaline Phosphatase - metabolism
Biomedical and Life Sciences
Biomedicine
Blood Platelets - physiology
Cell Biology
Cell Differentiation
Cell Extracts
Cells, Cultured
Enzyme Induction
Humans
Internal Medicine
Laboratory Medicine
Mesenchymal Stromal Cells - physiology
Osteogenesis
Osteopontin - genetics
Osteopontin - metabolism
Pathology
Stem cells
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Summary:We studied the effect of platelet releasate on osteogenic differentiation of human mesenchymal BM stem cells. Histological staining and reverse transcription PCR analysis showed that addition of platelet releasate to osteogenic differentiation medium stimulated the formation of calcium ossificates and alkaline phosphatase and increased expression of osteopontin and alkaline phosphatase genes.
ISSN:0007-4888
1573-8221
DOI:10.1007/s10517-014-2396-1