Characteristics of New Monomolecular Chimeric T-Cell Receptors to Carcinoembryonic Antigen

We described two original genetic constructs encoding chimeric monomolecular T-cell receptors, where the effector T-cell receptor fragment was linked with the antigen-recognizing part consisting of two variable fragments of two different antibodies to carcinoembryonic antigen. Following transfection...

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Bibliographic Details
Published in:Bulletin of experimental biology and medicine 2013-11, Vol.156 (1), p.165-171
Main Authors: Bozhenko, V. K., Shramova, E. I., Shishkin, A. M., Ivanov, A. V., Khokhlova, E. V., Lebedin, Yu. S., Shkoporov, A. N.
Format: Article
Language:English
Subjects:
Biomedical and Life Sciences
Biomedicine
Carcinoembryonic Antigen - immunology
CEA (Oncology)
Cell Biology
Cell Survival
Cytotoxicity, Immunologic
Genetically modified organisms
HCT116 Cells
HEK293 Cells
HT29 Cells
Humans
Immunotherapy
Internal Medicine
Laboratory Medicine
Neoplasms - immunology
Neoplasms - therapy
Pathology
Receptors, Antigen, T-Cell - genetics
Receptors, Antigen, T-Cell - metabolism
Recombinant Fusion Proteins - genetics
Recombinant Fusion Proteins - metabolism
T cells
Transfection
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Summary:We described two original genetic constructs encoding chimeric monomolecular T-cell receptors, where the effector T-cell receptor fragment was linked with the antigen-recognizing part consisting of two variable fragments of two different antibodies to carcinoembryonic antigen. Following transfection, these receptors were expressed on the cell surface and bound carcinoembryonic antigen. Human peripheral blood lymphocytes transfected with the above constructs demonstrated high cytotoxic activity against HCT116 cells expressing carcinoembryonic antigen.
ISSN:0007-4888
1573-8221
DOI:10.1007/s10517-013-2302-2