Dominant-negative Retinoic Acid Receptors Elicit Epidermal Defects through a Non-canonical Pathway

• Published in: The Journal of biological chemistry 2005-01, Vol.280 (4), p.3012-3021
• Main Authors: Chen, Chang Feng, Lohnes, David
• Format: Article
• Language: English
• Subjects: Animals; COS Cells; DNA - metabolism; DNA, Complementary - metabolism; Dose-Response Relationship, Drug; Down-Regulation; Epidermis - metabolism; Epidermis - pathology; Genes, Dominant; Immunohistochemistry; Keratinocytes - metabolism; Keratins - metabolism; Mice; Mice, Transgenic; Models, Genetic; Mutagenesis, Site-Directed; Mutation; Phenotype; Phosphoproteins - genetics; Promoter Regions, Genetic; Protein Binding; Receptors, Retinoic Acid - genetics; Receptors, Retinoic Acid - physiology; Retinoic Acid Receptor alpha; Signal Transduction; Time Factors; Trans-Activators - genetics; Transfection; Transgenes
• ISSN: 0021-9258; 1083-351X
• DOI: 10.1074/jbc.M411522200

Previous work has shown that a dominant-negative retinoic acid receptor α (dnRARα), expressed under the K14 promoter, causes severe epidermal defects. Similar defects are, however, not seen in RARαγ double null mutant mice, which lack the entire complement of RARs expressed in the epidermis. To investigate the mechanism of action of these dominant-negative receptors, dnRARα or a DNA binding-deficient variant, dnRARαDBD, were targeted to the basal epidermis. Expression of either receptor type led to similar epidermal phenotypes suggesting that both RAR mutants acted through a common mechanism. The epidermal phenotype was reminiscent of defects seen in p63-/- mice. Consistent with this, reduced p63 expression was observed in transgenic offspring expressing either RAR mutant, suggesting that down-regulation of p63 might underlie the effects of these receptors on epidermal development. By contrast, expression of p63 in the epidermis of RARαγ-/- offspring was unaffected, indicating that RARs were not essential for p63 expression. These findings suggest that dnRARs may impact on epidermal development through one or more non-canonical pathways, which are independent of receptor-DNA interaction.