Subunit composition of kainate receptors in hippocampal interneurons

Kainate receptor activation affects GABAergic inhibition in the hippocampus by mechanisms that are thought to involve the GluR5 subunit. We report that disruption of the GluR5 subunit gene does not cause the loss of functional KARs in CA1 interneurons, nor does it prevent kainate-induced inhibition...

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Bibliographic Details
Published in:Neuron (Cambridge, Mass.) Mass.), 2000-11, Vol.28 (2), p.475-484
Main Authors: Mulle, C, Sailer, A, Swanson, G T, Brana, C, O'Gorman, S, Bettler, B, Heinemann, S F
Format: Article
Language:English
Subjects:
Animals
Cells, Cultured
Crosses, Genetic
Dose-Response Relationship, Drug
Down-Regulation - drug effects
Excitatory Amino Acid Antagonists
gamma-Aminobutyric Acid - metabolism
GluK2 Kainate Receptor
GluR5 protein
GluR6 protein
Hippocampus - cytology
Hippocampus - drug effects
Hippocampus - metabolism
Interneurons - cytology
Interneurons - drug effects
Interneurons - metabolism
Kainate receptors
Kainic Acid - metabolism
Kainic Acid - pharmacology
Mice
Mice, Inbred Strains
Mice, Knockout
Neural Inhibition - drug effects
Neural Inhibition - physiology
Neuromuscular Depolarizing Agents - pharmacology
Neurons, Afferent - drug effects
Neurons, Afferent - metabolism
Patch-Clamp Techniques
Protein Subunits
Pyramidal Cells - cytology
Pyramidal Cells - drug effects
Pyramidal Cells - metabolism
Receptors, AMPA - antagonists & inhibitors
Receptors, Kainic Acid - deficiency
Receptors, Kainic Acid - genetics
Receptors, Kainic Acid - metabolism
Synaptic Transmission - drug effects
Synaptic Transmission - physiology
Tetrodotoxin - pharmacology
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Summary:Kainate receptor activation affects GABAergic inhibition in the hippocampus by mechanisms that are thought to involve the GluR5 subunit. We report that disruption of the GluR5 subunit gene does not cause the loss of functional KARs in CA1 interneurons, nor does it prevent kainate-induced inhibition of evoked GABAergic synaptic transmission onto CA1 pyramidal cells. However, KAR function is abolished in mice lacking both GluR5 and GluR6 subunits, indicating that KARs in CA1 stratum radiatum interneurons are heteromeric receptors composed of both subunits. In addition, we show the presence of presynaptic KARs comprising the GluR6 but not the GluR5 subunit that modulate synaptic transmission between inhibitory interneurons. The existence of two separate populations of KARs in hippocampal interneurons adds to the complexity of KAR localization and function.
ISSN:0896-6273
DOI:10.1016/S0896-6273(00)00126-4