Calcium and voltage mapping in hiPSC-CM monolayers

Graphical abstract Electrical activation maps of spontaneous and electrically paced propagation in human induced pluripotent stem cell-derived cardiomyocyte (hiPSC-CM) monolayers. (A) Spontaneous activity in dish 1. The spatial distribution of the activation timing, derived from maximum voltage upst...

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Bibliographic Details
Published in:Cell calcium (Edinburgh) 2016-03, Vol.59 (2-3), p.84-90
Main Author: Herron, Todd J
Format: Article
Language:English
Subjects:
Action
Advanced Basic Science
Calcium - metabolism
Calcium impulse
Calcium Signaling
Heart Conduction System
Human cardiac monolayers
Humans
Induced Pluripotent Stem Cells - cytology
Induced Pluripotent Stem Cells - metabolism
Myocytes, Cardiac - cytology
Myocytes, Cardiac - metabolism
Potential mapping
Stem cells
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Summary:Graphical abstract Electrical activation maps of spontaneous and electrically paced propagation in human induced pluripotent stem cell-derived cardiomyocyte (hiPSC-CM) monolayers. (A) Spontaneous activity in dish 1. The spatial distribution of the activation timing, derived from maximum voltage upstroke velocity, with isochronal lines at each 2-ms interval. (B) Electrically stimulated (at the monolayer center) activity in dish 1. The spatial distribution of the activation timing, derived from maximum voltage upstroke velocity, with isochronal lines at each 2-ms interval. The 2 white circles are separated by a 5-mm distance. At a pacing frequency of 2 Hz, it took 24.9 ± 2.3 ms (from 3 stimulations) for the activation wave to propagate from the inner to the outer circle. This translates to a conduction velocity of 20.1 cm/s−1 for this monolayer. Scale bar: 5 mm C, hiPSC-CM monolayers contain atrial (MLC2a+) and ventricular (MLC2v+) cardiomyocytes.
ISSN:0143-4160
1532-1991
DOI:10.1016/j.ceca.2016.02.004