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Influences of acidic reaction and hydrolytic conditions on monosaccharide composition analysis of acidic, neutral and basic polysaccharides
•A hydrolytic rule for monosaccharide composition analysis was developed.•The stability of 10 different monosaccharides was compared.•The basic amino sugars were hard to release but most stable.•The acidic sugars (uronic acids) were easy to release but most unstable.•The release and stability of neu...
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Published in: | Carbohydrate polymers 2016-06, Vol.143, p.296-300 |
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Main Authors: | , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | •A hydrolytic rule for monosaccharide composition analysis was developed.•The stability of 10 different monosaccharides was compared.•The basic amino sugars were hard to release but most stable.•The acidic sugars (uronic acids) were easy to release but most unstable.•The release and stability of neutral sugars were in between acidic and basic sugars.
Monosaccharide composition analysis is important for structural characterization of polysaccharides. To investigate the influences of acidic reaction and hydrolytic conditions on monosaccharide composition analysis of polysaccharides, we chose alginate, starch, chitosan and chondroitin sulfate as representative of acidic, neutral, basic and complex polysaccharides to compare the release degree of monosaccharides under different hydrolytic conditions. The hydrolysis stability of 10 monosaccharide standards was also explored. Results showed that the basic sugars were hard to release but stable, the acidic sugars (uronic acids) were easy to release but unstable, and the release and stability of neutral sugars were in between acidic and basic sugars. In addition, the hydrolysis process was applied to monosaccharide composition analysis of Hippocampus trimaculatus polysaccharide and the appropriate hydrolytic condition was accorded with that of the above four polysaccharides. Thus, different hydrolytic conditions should be used for the monosaccharide composition analysis of polysaccharides based on their structural characteristics. |
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ISSN: | 0144-8617 1879-1344 |
DOI: | 10.1016/j.carbpol.2016.02.023 |