Inducible expression of a Phytolacca heterotepala ribosome-inactivating protein leads to enhanced resistance against major fungal pathogens in tobacco

ABSTRACT Plant genetic engineering has long been considered a valuable tool to fight fungal pathogens because it would limit the economically costly and environmentally undesirable chemical methods of disease control. Ribosome-inactivating proteins (RIPs) are potentially useful for plant defense con...

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Bibliographic Details
Published in:Phytopathology 2005-02, Vol.95 (2), p.206-215
Main Authors: Corrado, G, Delli Bovi, P, Ciliento, R, Gaudio, L, Di Maro, A, Aceto, S, Lorito, M, Rao, R
Format: Article
Language:English
Subjects:
Alternaria alternata
Biological and medical sciences
Botrytis cinerea
complementary DNA
disease resistance
Fundamental and applied biological sciences. Psychology
fungal diseases of plants
Fungal plant pathogens
gene expression
gene transfer
genes
molecular sequence data
Nicotiana tabacum
nucleotide sequences
pathogenicity
Phytolacca
Phytolacca heterotepala
Phytopathology. Animal pests. Plant and forest protection
plant breeding
plant pathogenic fungi
plant proteins
ribosomes
tobacco
transgenic plants
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Summary:ABSTRACT Plant genetic engineering has long been considered a valuable tool to fight fungal pathogens because it would limit the economically costly and environmentally undesirable chemical methods of disease control. Ribosome-inactivating proteins (RIPs) are potentially useful for plant defense considering their antiviral and antimicrobial activities but their use is limited by their cytotoxic activity. A new gene coding for an RIP isolated from leaves of Phytolacca heterotepala was expressed in tobacco under the control of the wound-inducible promoter of the bean polygalacturonase-inhibiting protein I gene to increase resistance against different fungal pathogens, because an individual RIP isolated from P. heterotepala showed direct antifungal toxicity. Phenotypically normal transgenic lines infected with Alternaria alternata and Botrytis cinerea showed a significant reduction of leaf damage while reverse transcription-polymerase chain reaction and western analysis indicated the expression of the RIP transgene upon wounding and pathogen attack. This work demonstrates that use of a wound-inducible promoter is useful to limit the accumulation of antimicrobial phytotoxic proteins only in infected areas and that the controlled expression of the PhRIP I gene can be very effective to control fungal pathogens with different phytopathogenic actions.
ISSN:0031-949X
1943-7684
DOI:10.1094/PHYTO-95-0206