A simple and rapid method for lithium acetate-mediated transformation of Kluyveromyces marxianus cells

Efficient plasmid transformation of Kluyveromyces marxianus cells of 1.9 × 10^sup 3^ transformant μg^sup -1^ DNA with an episomal plasmid was achieved by the use of a simple lithium acetate method with the addition of 10 mM DTT and an increased heat shock temperature of 47 °C. This method is shown t...

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Bibliographic Details
Published in:World journal of microbiology & biotechnology 2000-01, Vol.16 (7), p.653-654
Main Authors: ANTUNES, Daiane Felberg, GALVAO DE SOUZA, Claudio JR, DE MORAIS JUNIOR, Marcos Antonio
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biotechnology
Fundamental and applied biological sciences. Psychology
Gene transfer
Genetic engineering
Genetic technics
Kluyveromyces marxianus
Lithium
lithium acetate
Methods. Procedures. Technologies
Plasmids
Synthetic digonucleotides and genes. Sequencing
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Summary:Efficient plasmid transformation of Kluyveromyces marxianus cells of 1.9 × 10^sup 3^ transformant μg^sup -1^ DNA with an episomal plasmid was achieved by the use of a simple lithium acetate method with the addition of 10 mM DTT and an increased heat shock temperature of 47 °C. This method is shown to be also efficient for replicative plasmids. Therefore, we suggest its use as a routine method to transform K. marxianus cells.[PUBLICATION ABSTRACT]
ISSN:0959-3993
1573-0972
DOI:10.1023/A:1008984403732