TAG-1-Deficient Mice Have Marked Elevation of Adenosine A1 Receptors in the Hippocampus

TAG-1 is a neural recognition molecule in the immunoglobulin superfamily that is predominantly expressed in the developing brain. Several lines of evidence suggest that TAG-1 is involved in the outgrowth, guidance, and fasciculation of neurites. To directly assess the function of TAG-1 in vivo, we h...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2001-02, Vol.281 (1), p.220-226
Main Authors: Fukamauchi, Fumihiko, Aihara, Okihiko, Wang, Yi-Jun, Akasaka, Keiko, Takeda, Yasuo, Horie, Masao, Kawano, Hitoshi, Sudo, Katsuko, Asano, Masahide, Watanabe, Kazutada, Iwakura, Yoichiro
Format: Article
Language:English
Subjects:
adenosine receptor
Animals
Antibodies - metabolism
Blotting, Southern
Blotting, Western
Cell Adhesion
Cell Adhesion Molecules, Neuronal
Cerebellum - metabolism
Contactin 2
Embryo, Mammalian - metabolism
Gene Deletion
gene targeting
Hippocampus - metabolism
hippocampus, convulsive behavior
immunoglobulin superfamily
Immunohistochemistry
Membrane Glycoproteins - genetics
Membrane Glycoproteins - physiology
Mice
Mice, Knockout
Models, Genetic
Mutagenesis, Site-Directed
Rabbits
Receptors, Purinergic P1 - biosynthesis
Recombination, Genetic
Seizures - genetics
Spinal Cord - metabolism
Stem Cells - metabolism
TAG-1
Up-Regulation
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Summary:TAG-1 is a neural recognition molecule in the immunoglobulin superfamily that is predominantly expressed in the developing brain. Several lines of evidence suggest that TAG-1 is involved in the outgrowth, guidance, and fasciculation of neurites. To directly assess the function of TAG-1 in vivo, we have generated mice with a deletion in the gene encoding TAG-1 using homologous recombination in embryonic stem cells. Gross morphological analysis of the cerebellum, the spinal cord, and the hippocampus appeared normal in TAG-1-deficient mice. However, TAG-1 (−/−) mice showed the upregulation of the adenosine A1 receptors determined by [3H]cyclopentyl-1,3-dipropylxanthine in the hippocampus, and their greater sensitivity to convulsant stimuli than that in TAG-1 (+/+) mice. We suspect that the subtle changes in neural plasticity induced by TAG-1 deficiency during development cause the selective vulnerability of specific brain regions and the epileptogenicity in TAG-1 (−/−) mice.
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.2001.4334