Expression of multiple insecticidal genes confers broad resistance against a range of different rice pests

We report the simultaneous introduction of three insecticidal genes (the Bt genes cry1Ac and cry2A, and the snowdrop lectin gene gna) into commercially important indica rice varieties M7 and Basmati 370, by particle bombardment. Transgenic plants expressed Cry1Ac, Cry2A and GNA at different levels,...

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Bibliographic Details
Published in:Molecular breeding 2001-01, Vol.7 (1), p.85-93
Main Authors: Shahina Bano Maqbool, Sheikh Riazuddin, Nguyen, Thi Loc, Gatehouse, Angharad MR, Gatehouse, John A, Christou, Paul
Format: Article
Language:English
Subjects:
Bioassays
Bombardment
Borers
Cnaphalocrocis medinalis
cry1Ac gene
Cry1Ac toxin
cry2A gene
Delphacidae
Gene expression
Gene transfer
Genes
gna gene
Insecticide resistance
Insects
Leaves
Molecular biology
Nilaparvata lugens
Oryza sativa
Oryza sativa indica
Particle bombardment
Pest resistance
Pests
Plant biology
Plant protection
Plants (botany)
Pyralidae
Reduction
Rice
Scirpophaga incertulas
Snowdrop lectin gene
Survival
Technology transfer
Transgenes
Transgenic plants
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Summary:We report the simultaneous introduction of three insecticidal genes (the Bt genes cry1Ac and cry2A, and the snowdrop lectin gene gna) into commercially important indica rice varieties M7 and Basmati 370, by particle bombardment. Transgenic plants expressed Cry1Ac, Cry2A and GNA at different levels, either singly or in combination at 0.03–1%, 0.01–0.5% and 0.01–2.5% of total soluble protein, respectively. The transgenes showed stable transmission and expression, and R1 transgenic plants provided significant (p
ISSN:1380-3743
1572-9788
DOI:10.1023/A:1009644712157