Genetic analysis of an incomplete bio operon in a biotin auxotrophic strain of Bacillus subtilis natto OK2

We describe the genetic analysis of the bio operon of the biotin auxotrophic Bacillus subtilis natto OK2 strain. The OK2 strain would only cross-feed with the Escherichia coli bioB mutant and also grew well in medium containing dethiobiotin. Sequencing analysis revealed two significant genetic alter...

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Bibliographic Details
Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2004-03, Vol.68 (3), p.739-742
Main Authors: Sasaki, M. (Ibaraki Univ., Ami (Japan). Faculty of Agriculture), Kawamura, F, Kurusu, Y
Format: Article
Language:English
Subjects:
AUXOTROPHY
BACILLUS SUBTILIS
Bacillus subtilis - genetics
Bacillus subtilis - growth & development
Bacillus subtilis - metabolism
Bacillus subtilis natto
bioB
Biological and medical sciences
BIOSYNTHESIS
BIOTIN
Biotin - biosynthesis
Biotin - genetics
biotin operon
Escherichia coli
Fundamental and applied biological sciences. Psychology
Gene Deletion
Gene Order - genetics
GENES
Genetic Complementation Test
GENETICS
NUCLEOTIDE SEQUENCE
Operon - genetics
Point Mutation
Sequence Analysis, DNA
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Summary:We describe the genetic analysis of the bio operon of the biotin auxotrophic Bacillus subtilis natto OK2 strain. The OK2 strain would only cross-feed with the Escherichia coli bioB mutant and also grew well in medium containing dethiobiotin. Sequencing analysis revealed two significant genetic alterations in the bioW and bioF genes within the bio operon of the OK2 strain. Complementation analysis with B. subtilis 168 bio mutants demonstrated that only the bioB gene could complement, but other bio operon genes could not. A bio + transformant, isolated from an OK2 strain, has biotin autotrophy.
ISSN:0916-8451
1347-6947
DOI:10.1271/bbb.68.739