4-tert-octylphenol and 17β-estradiol applied by feeding to flounder Platichthys flesus: induction of vitellogenin and accumulation in tissues

• Published in: Marine environmental research 2002-09, Vol.54 (3-5), p.729-733
• Main Authors: Madsen, Louise L, Korsgaard, Bodil, Bjerregaard, Poul
• Format: Article
• Language: English
• Subjects: 4-Tert-octylphenol; Agnatha. Pisces; Alkylphenols; Animal, plant and microbial ecology; Applied ecology; Biological and medical sciences; Ecotoxicology, biological effects of pollution; Effects of pollution and side effects of pesticides on vertebrates; Flounder; Fundamental and applied biological sciences. Psychology; Marine; Platichthys flesus; Vitellogenin
• ISSN: 0141-1136; 1879-0291
• DOI: 10.1016/S0141-1136(02)00111-3

In order to simulate a possible natural administration route of xenoestrogens male flounder Platichthys flesus were exposed via the diet to the alkylphenol 4-tert-octylphenol. Treatment with 4-tert-octylphenol (10, 50 and 100 mg OP kg−1 BW) or 17β-estradiol (0.05 mg kg−1 BW) every second day during a period of 11 days resulted in a significant increase in plasma vitellogenin (Vtg) concentrations. The induction of Vtg was greatest in the fish receiving 50 mg OP kg−1 BW. A significant accumulation of 4-tert-octylphenol was found in liver and muscle tissue of the OP treated groups. The tissue concentrations of 4-tert-octylphenol and the plasma vitellogenin concentration were positively correlated. The results show that 4-tert-octylphenol accumulates in liver and muscles of flounder P. flesus, and exerts estrogenic effects such as vitellogenin induction.