Oxygen Distribution in Wetland Plant Roots and Permeability Barriers to Gas-exchange with the Rhizosphere: a Microelectrode and Modelling Study with Phragmites australis

• Published in: Annals of botany 2000-09, Vol.86 (3), p.687-703
• Main Authors: Armstrong, W., Cousins, D., Armstrong, J., Turner, D.W., Beckett, P.M.
• Format: Article
• Language: English
• Subjects: Cell walls; Cylinders; Electrodes; Epidermal cells; Freshwater; modelling; Oxygen; oxygen permeability; Phragmites; Phragmites australis; Physiology and Anatomy of Roots in Wetland Environments; Plant roots; Plants; Rhizosphere; Rice; Transport; Transport, oxygen permeability, Phragmites, wetland roots, rhizosphere, modelling; wetland roots; Wetlands
• ISSN: 0305-7364; 1095-8290
• DOI: 10.1006/anbo.2000.1236

Adventitious roots of intact Phragmites plantlets were secured horizontally 2–3mm below the surface of an oxygen-depleted fluid agar across which oxygen-free nitrogen was gently streamed to create a constant oxygen sink; the leafy shoot was fully exposed to air. Radial oxygen profiles through rhizosphere and root at different distances from the apex were obtained polarographically using Clark-type bevelled microelectrodes servo-driven in steps of 10μm (root) or 10–50μm (rhizosphere). The pattern of radial oxygen loss (ROL) typical of wetland plants, viz. high at the apex and declining sharply sub-apically, was related to synergism between ROL, and oxygen consumption and increasing impedance to diffusion within the epidermal/hypodermal cylinder rather than to a surface resistance. The smallest oxygen deficit (2kPa) to develop across the 80μm thick epidermal/hypodermal cylinder was within the apical 10mm and was consistent with tissue oxygen diffusivities similar to water. At 100mm from the apex, consumption and impedance had increased the deficit to about 15kPa and reduced ROL almost to zero. The developing impedance within the epidermal/hypodermal cylinder was least in cell layers immediately adjoining the cortex and increased most in the hypodermal cell layer abutting the epidermis. The sub-apical decline in ROL appeared to coincide with the appearance of aerenchyma in the cortex but thin walled ‘passage areas’ (windows) in the hypodermal/epidermal cylinder persisted locally and remained leaky to oxygen to some degree. It is through these windows that lateral roots emerge and the cortex in line with the windows remains non-aerenchymatous. The radial and longitudinal oxygen profiles were consistent with modelling predictions. The shapes of the stelar oxygen profiles were consistent with a higher oxygen demand in the outer region (viz. pericycle, phloem, protoxylem and early metaxylem cylinder) than in the inner core (late metaxylem cylinder and medulla), but the deficits were relatively small (⩽3kPa) and consistent with minimal wall thickening in the endodermis and narrowness of stele. The possible relevance of the results to entry of methane and other products from the rhizosphere into root and to the mechanism of aerenchyma formation are discussed.