Persistence of benzo[a]pyrene–DNA adducts in hematopoietic tissues and blood of the mummichog, Fundulus heteroclitus

The formation and persistence of benzo[a]pyrene (B[a]P)–DNA adducts were investigated in blood, liver and two hematopoietic tissues (anterior kidney and spleen) of the mummichog ( Fundulus heteroclitus). Fish were injected with a single, sublethal dose of B[a]P (12 mg/kg body weight) and sampled fro...

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Bibliographic Details
Published in:Aquatic toxicology 2001-05, Vol.52 (3), p.319-328
Main Authors: Rose, Wendy L, French, Barbara L, Reichert, William L, Faisal, Mohamed
Format: Article
Language:English
Subjects:
Agnatha. Pisces
Animal, plant and microbial ecology
Animals
Applied ecology
Benzo(a)pyrene
Benzo(a)pyrene - analysis
Biological and medical sciences
Cytochrome P-450 Enzyme System - biosynthesis
DNA adducts
DNA Adducts - analysis
Ecotoxicology, biological effects of pollution
Effects of pollution and side effects of pesticides on vertebrates
Enzyme Induction
Fundamental and applied biological sciences. Psychology
Fundulus heteroclitus
Hematopoiesis
Hematopoietic Stem Cells - chemistry
Hematopoietic tissues
Kidney - chemistry
Killifishes - metabolism
Liver - chemistry
Spleen - chemistry
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Summary:The formation and persistence of benzo[a]pyrene (B[a]P)–DNA adducts were investigated in blood, liver and two hematopoietic tissues (anterior kidney and spleen) of the mummichog ( Fundulus heteroclitus). Fish were injected with a single, sublethal dose of B[a]P (12 mg/kg body weight) and sampled from 8 to 96 days post-injection. 32P-Postlabeling analysis and storage phosphor imaging were used to resolve and quantify hydrophobic DNA adducts. One major DNA adduct was present in each of the examined tissues at all sampling times. This adduct had similar chromatographic characteristics to those of the adduct standard, 7 R,8 S,9 S-trihydroxy-10 S-( N 2-deoxyguanosyl-3′-phosphate)-7,8,9,10-tetrahydro-benzo[a]pyrene (B[a]PDE-dG). Minor DNA adduct spots, representing less than 2% of the total DNA adducts, were observed in some liver, anterior kidney and spleen samples for up to 32 days post-injection. The B[a]P–DNA adducts reached maximal levels at 32 days post-injection and persisted for at least 96 days in all examined tissues. B[a]P–DNA adduct levels were significantly higher in the liver and anterior kidney than in the spleen from 16 to 96 days ( P
ISSN:0166-445X
1879-1514
DOI:10.1016/S0166-445X(00)00125-9