Patterns of GAP-43 (B-50) expression and accumulation in subchronic acrylamide neurotoxicity

The expression and accumulation of GAP‐43 was characterized by immunoblot, immunohistochemistry, and in situ hybridization in the dorsal root ganglia and sciatic nerve of rats exposed to 0.05% acrylamide in drinking water for 14 days. Animals were sacrificed on 2, 10, 14, 21, 28, 35, or 80 days afte...

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Bibliographic Details
Published in:Neuroscience research communications 2000-11, Vol.27 (3), p.199-212
Main Authors: Reagan, Katherine E., Jensen, Karl F., Friedman, Marvin A., Abou-Donia, Mohamed B.
Format: Article
Language:English
Subjects:
axon
degeneration
dorsal root ganglia
GAP-43 protein
nerve crush
peripheral neuropathy
regeneration
sciatic nerve
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Summary:The expression and accumulation of GAP‐43 was characterized by immunoblot, immunohistochemistry, and in situ hybridization in the dorsal root ganglia and sciatic nerve of rats exposed to 0.05% acrylamide in drinking water for 14 days. Animals were sacrificed on 2, 10, 14, 21, 28, 35, or 80 days after the initiation of exposure. GAP‐43 mRNA expression in the dorsal root ganglia increased at 14 days and GAP‐43 protein in sciatic nerve increased at 24 days. The peak of GAP‐43 expression corresponded to the time of the maximal rate of clinical recovery, consistent with the hypothesis that GAP‐43 is involved aspects of neurological recovery in addition to axonal regeneration.
ISSN:0893-6609
1520-6769
DOI:10.1002/1520-6769(200011/12)27:3<199::AID-NRC7>3.0.CO;2-X