The effect of rapamycin on TGFβ1 and MMP1 expression in a rabbit model of urethral stricture

Purpose To investigate the effect of rapamycin on TGFβ1 and MMP1 expression in a rabbit model of urethral stricture. Methods Twenty-four adult New Zealand male rabbits underwent an electrocoagulation of the bulbar urethra with a 13Fr pediatric resectoscope. Then rabbits were randomly divided into th...

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Bibliographic Details
Published in:International urology and nephrology 2016-05, Vol.48 (5), p.717-723
Main Authors: Huang, S. L., Fu, D. L., Li, H. C., Zhang, P., Chong, T.
Format: Article
Language:English
Subjects:
Animals
Antibiotics, Antineoplastic - pharmacology
Collagen - analysis
Disease Models, Animal
Fibrosis
Gene Expression - drug effects
Male
Matrix Metalloproteinase 1 - analysis
Matrix Metalloproteinase 1 - genetics
Medicine
Medicine & Public Health
Nephrology
Protein Biosynthesis - drug effects
Rabbits
RNA, Messenger - analysis
Sirolimus - pharmacology
Transforming Growth Factor beta1 - analysis
Transforming Growth Factor beta1 - genetics
Urethral Stricture - genetics
Urethral Stricture - metabolism
Urethral Stricture - pathology
Urology
Urology - Original Paper
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Summary:Purpose To investigate the effect of rapamycin on TGFβ1 and MMP1 expression in a rabbit model of urethral stricture. Methods Twenty-four adult New Zealand male rabbits underwent an electrocoagulation of the bulbar urethra with a 13Fr pediatric resectoscope. Then rabbits were randomly divided into three groups: (1) normal control group: normal saline (NS), (2) the vehicle control group: dimethyl sulfoxide (DMSO), and (3) the treatment group: effective-dose rapamycin in DMSO (Ra), with 12, 6, and 6 rabbits in each group, respectively. Drugs were given by urethral irrigation daily for 4 weeks. Urethral tissue was harvested for histological and molecular analyses. TGFβ1 and MMP1 expression levels were evaluated by real-time quantitative PCR and immunohistochemistry. Results Ten, six, and six rabbits were evaluated finally in Ra, DMSO, and NS group, respectively. Histological examination revealed the distribution of fibrosis and the degree of collagen deposition in the Ra group were smaller and slighter than the two control groups. Collagen content was significantly less in the Ra group than in the DMSO group ( P  
ISSN:0301-1623
1573-2584
DOI:10.1007/s11255-016-1227-x