Expression of recombinant organophosphorus hydrolase in the original producer of the enzyme, Sphingobium fuliginis ATCC 27551

The plasmid encoding His-tagged organophosphorus hydrolase (OPH) cloned from Sphingobium fuliginis was modified to be transferred back to this bacterium. The replication function of S. amiense plasmid was inserted at downstream of OPH gene, and S. fuliginis was transformed with this plasmid. The tra...

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Bibliographic Details
Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2016-05, Vol.80 (5), p.1024-1026
Main Authors: Nakayama, Kosuke, Ohmori, Takeshi, Ishikawa, Satoshi, Iwata, Natsumi, Seto, Yasuo, Kawahara, Kazuyoshi
Format: Article
Language:English
Subjects:
Aryldialkylphosphatase - genetics
Aryldialkylphosphatase - metabolism
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Biodegradation, Environmental
DNA Replication
DNA, Bacterial - genetics
DNA, Bacterial - metabolism
Escherichia coli - enzymology
Escherichia coli - genetics
Gene Expression
Histidine - genetics
Histidine - metabolism
Humans
nerve agent
Nerve Agents - metabolism
Oligopeptides - genetics
Oligopeptides - metabolism
Organophosphorus Compounds - metabolism
organophosphorus hydrolase
paraoxon
Plasmids - chemistry
Plasmids - metabolism
Recombinant Fusion Proteins - genetics
Recombinant Fusion Proteins - metabolism
Sphingobium amiense
Sphingobium fuliginis
Sphingomonadaceae - enzymology
Sphingomonadaceae - genetics
Transformation, Bacterial
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Summary:The plasmid encoding His-tagged organophosphorus hydrolase (OPH) cloned from Sphingobium fuliginis was modified to be transferred back to this bacterium. The replication function of S. amiense plasmid was inserted at downstream of OPH gene, and S. fuliginis was transformed with this plasmid. The transformant produced larger amount of active OPH with His-tag than E. coli.
ISSN:0916-8451
1347-6947
DOI:10.1080/09168451.2015.1123606