Direct visualization of replication dynamics in early zebrafish embryos

We analyzed DNA replication in early zebrafish embryos. The replicating DNA of whole embryos was labeled with the thymidine analog 5-ethynyl-2ʹ-deoxyuridine (EdU), and spatial regulation of replication sites was visualized in single embryo-derived cells. The results unveiled uncharacterized replicat...

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Bibliographic Details
Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2016-05, Vol.80 (5), p.945-948
Main Authors: Kuriya, Kenji, Higashiyama, Eriko, Avşar-Ban, Eriko, Okochi, Nanami, Hattori, Kaede, Ogata, Shin, Takebayashi, Shin-ichiro, Ogata, Masato, Tamaru, Yutaka, Okumura, Katsuzumi
Format: Article
Language:English
Subjects:
Animals
Deoxyuridine - analogs & derivatives
Deoxyuridine - metabolism
DNA Replication
Embryo, Nonmammalian - metabolism
Embryo, Nonmammalian - ultrastructure
Embryonic Development - genetics
Microscopy, Fluorescence
replication foci
Staining and Labeling
Zebrafish - embryology
Zebrafish - genetics
zebrafish embryo
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Summary:We analyzed DNA replication in early zebrafish embryos. The replicating DNA of whole embryos was labeled with the thymidine analog 5-ethynyl-2ʹ-deoxyuridine (EdU), and spatial regulation of replication sites was visualized in single embryo-derived cells. The results unveiled uncharacterized replication dynamics during zebrafish early embryogenesis.
ISSN:0916-8451
1347-6947
DOI:10.1080/09168451.2016.1141039