AFLP-based PCR markers that differentiate spot and net forms of Pyrenophora teres

Specific polymerase chain reaction (PCR) primers were developed from amplified fragment length polymorphism (AFLP) fragments of Pyrenophora teres, the causal agent of net blotch on barley leaves. The primers were designed specifically to amplify DNA from P. teres f. teres (net form) and allow its di...

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Bibliographic Details
Published in:Plant pathology 2005-02, Vol.54 (1), p.66-73
Main Authors: Leisova, L, Kucera, L, Minarikova, V, Ovesna, J
Format: Article
Language:English
Subjects:
amplified fragment length polymorphism
barley
barley net blotch
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
fungal diseases of plants
genetic markers
Helminthosporium
Hordeum vulgare
host plants
leaf blotch
molecular sequence data
nucleotide sequences
PCR detection
Phytopathology. Animal pests. Plant and forest protection
plant pathogenic fungi
polymerase chain reaction
Pyrenophora teres
Pyrenophora teres f. maculata
Pyrenophora teres f. teres
SCAR markers
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Summary:Specific polymerase chain reaction (PCR) primers were developed from amplified fragment length polymorphism (AFLP) fragments of Pyrenophora teres, the causal agent of net blotch on barley leaves. The primers were designed specifically to amplify DNA from P. teres f. teres (net form) and allow its differentiation from P. teres f. maculata (spot form), which is morphologically very similar to P. teres f. teres in culture. The PCR amplification was carried out successfully from DNA extracted from fungal mycelium. The PCR assay was validated with 60 samples of Pyrenophora species. The amplification with four designed PCR primer pairs provided P. teres form‐specific products. No cross‐reaction was observed with DNA of several other species, such as P. tritici‐repentis, P. graminea and Helminthosporium sativum.
ISSN:0032-0862
1365-3059
DOI:10.1111/j.1365-3059.2005.01117.x