Growth and protopectinase production of Geotrichum klebahnii in batch and continuous cultures with synthetic media

ATCC 42397 produces a protopectinase (PPase-SE) with polygalacturonase (PGase) activity. The microorganism was aerobically cultivated in synthetic media. Glucose, fructose and xylose yielded the highest enzyme levels (10-11 PGase units ml^sup -1^). Galacturonic acid repressed enzyme production and n...

Full description

Saved in:
Bibliographic Details
Published in:Journal of industrial microbiology & biotechnology 2000-11, Vol.25 (5), p.260-265
Main Authors: CAVALITTO, S. F, HOURS, R. A, MIGNONE, C. F
Format: Article
Language:English
Subjects:
Acids
Biological and medical sciences
Biomass
Bioreactors
Biosynthesis
Biotechnology
Carbon
Chemical products
Enzymatic activity
Enzyme engineering
Enzymes
Fermentation
fructose
Fundamental and applied biological sciences. Psychology
Geotrichum klebahnii
Glucose
Glycerol
Methods. Procedures. Technologies
Microbiology
Morphology
Production of selected enzymes
protopectinase
Retention
Studies
Urea
xylose
Yeast
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:ATCC 42397 produces a protopectinase (PPase-SE) with polygalacturonase (PGase) activity. The microorganism was aerobically cultivated in synthetic media. Glucose, fructose and xylose yielded the highest enzyme levels (10-11 PGase units ml^sup -1^). Galacturonic acid repressed enzyme production and no growth was obtained with disaccharides and pectin. Specific enzyme activity obtained in an O^sub 2^-limited culture was similar to that found in nonlimited ones. A growth yield (Y^sub x/s^) of 0.49 g of cell dry weight per gram of glucose consumed was obtained in a typical batch bioreactor culture. Enzyme production was growth associated, and no major products other than biomass and CO^sub 2^ were detected. The volumetric enzyme activity reached a maximum around D=0.3-0.4 h^sup -1^ in glucose-limited continuous cultures. However, it varied strongly (together with microorganism morphology) even after retention times ≥8 at any D tested (0.035-0.44 h^sup -1^) though the rest of the culture variables remained fairly constant. No correlation between morphology and enzyme activity could be obtained. Enzyme production was poor in urea- and vitamin-limited continuous cultures. In all cases, biomass and CO^sub 2^ accounted for 100% of carbon recovery though Y^sub x/s^ values were different. Journal of Industrial Microbiology & Biotechnology (2000) 25, 260-265.[PUBLICATION ABSTRACT]
ISSN:1367-5435
1476-5535
DOI:10.1038/sj.jim.7000072