Effect of sludge fasting/feasting on growth of activated sludge cultures

• Published in: Water research (Oxford) 2001-03, Vol.35 (4), p.1029-1037
• Main Authors: Chen, Guang-Hao, Yip, Wai-Keung, Mo, Hau-Kwok, Liu, Yu
• Format: Article
• Language: English
• Subjects: activated sludge culture; Adenosine Triphosphate - metabolism; Aerobiosis; Anaerobiosis; Applied sciences; ATP; ATP content; Biological and medical sciences; Biological treatment of waters; Bioreactors; Biotechnology; Carbohydrate Metabolism; carbohydrate storage; Environment and pollution; Exact sciences and technology; Fundamental and applied biological sciences. Psychology; General purification processes; Industrial applications and implications. Economical aspects; low-ORP control; observed growth yield; Oxidation-Reduction; Pollution; Sewage - microbiology; sludge fasting/feasting; SOUR; Waste Disposal, Fluid - instrumentation; Waste Disposal, Fluid - methods; Wastewaters; Water treatment and pollution
• ISSN: 0043-1354; 1879-2448
• DOI: 10.1016/S0043-1354(00)00346-8

Reduction of excess sludge in an oxic-settling-anoxic (OSA) activated sludge process might be attributed to a “sludge fasting (insufficient food under an anoxic condition)/feasting (sufficient food under an oxic condition)” treatment. This paper was to examine this explanation by investigating both the sludge fasting/feasting phenomenon and the effect of a fasting/feasting treatment on sludge growth. In this study, five different activated sludge cultures cultivated using synthetic wastewater composed of mainly glucose and other necessary nutrients: (1) an aerobic batch culture, (2) an intermittently aerated batch culture, (3) an anoxic batch culture, (4) a continuous aerobic culture, and (5) an OSA culture, were employed. It was found that only the aerobic batch culture and the aerobic continuous culture are fastable when the oxidation reduction potential (ORP) level is below 100 mV under no-food condition during a 2-h fasting treatment, showing that both the biomass and carbohydrate storage of these two cultures were reduced after the treatment. When the fasted cultures were treated in a feasting environment, an accumulation of carbohydrate storage did not occur, while specific oxygen uptake rates (SOUR) showed a sharp increase. Both the substrate utilization and biomass growth rates were also accelerated. It was therefore confirmed that a sludge feasting did occur after a fasting treatment for the fastable cultures. However, an increase in sludge ATP content was not brought about by the feasting treatment. The sludge fasting/feasting treatment in this paper could not induce a reduction of the observed growth yield ( Y obs) in all the cultures cultivated with glucose-based synthetic wastewater.