A renewable amperometric immunosensor for phytohormone β-indole acetic acid assay

A rapid, convenient and accurate method using a sol–gel–alginate–carbon composite electrode (SACE) is described for the determination of β-indole acetic acid (IAA) in plant samples. The determination is based on an enzyme-linked competitive immunoreaction between free IAA and IAA labeled with HRP to...

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Bibliographic Details
Published in:Analytica chimica acta 2003-10, Vol.494 (1), p.177-185
Main Authors: Li, Jin, Xiao, Lang-Tao, Zeng, Guang-Ming, Huang, Guo-He, Shen, Guo-Li, Yu, Ru-Qin
Format: Article
Language:English
Subjects:
Amperometric immunosensor
Analytical chemistry
Biological and medical sciences
Biosensors
Biotechnology
Chemistry
Electrochemical methods
Exact sciences and technology
Fundamental and applied biological sciences. Psychology
Hapten–protein conjugation
Immunoassay
Methods. Procedures. Technologies
Oryza sativa
Various methods and equipments
β-Indole acetic acid
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Summary:A rapid, convenient and accurate method using a sol–gel–alginate–carbon composite electrode (SACE) is described for the determination of β-indole acetic acid (IAA) in plant samples. The determination is based on an enzyme-linked competitive immunoreaction between free IAA and IAA labeled with HRP to bind on the anti-IAA IgG immobilized on the SACE surface. The response signal expressed as percentage current reduction (CR%, y) is linearly related to the logarithm of the concentration of IAA ( x) in range of 5–500 μg ml −1 with a regression equation of the form y=37.80 x−22.47 and correlation coefficient of 0.9922. The immunosensor was used to determine the IAA content in the hybrid rice grain samples taken in the growing period.
ISSN:0003-2670
1873-4324
DOI:10.1016/S0003-2670(03)00988-7